CELL SPECIFIC DISTRIBUTION OF LH HCG RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN RAT TESTICULAR LEYDIG-CELLS

被引:3
作者
BHALLA, VK
BEHZADIAN, MA
GEORGE, PE
HOWARD, EF
MAHESH, VB
ABNEY, TO
机构
[1] MED COLL GEORGIA,DEPT OBSTET & GYNECOL,AUGUSTA,GA 30912
[2] MED COLL GEORGIA,DEPT CELL BIOL,AUGUSTA,GA 30912
[3] MED COLL GEORGIA,DEPT ANAT,AUGUSTA,GA 30912
[4] MED COLL GEORGIA,DEPT BIOCHEM & MOLEC BIOL,AUGUSTA,GA 30912
关键词
D O I
10.1210/en.131.5.2485
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
By using four different cell isolation procedures, we previously identified two morphologically and biochemically distinct Leydig cell populations in rat testis. The light cells were vacuolated and bound I-125-labeled human choriogonadotropin (hCG) with high affinity but upon hCG stimulation in vitro, cAMP and testosterone production by then cells were minimal. On the other hand, the heavier cells displayed typical Leydig cell morphology and bound very little hCG but vigorously produced cAMP and testosterone (Browne, E.S., Bhalla, V.K.. 1991, J. Androl. 12:132-139). This study examines the distribution of LH/hCG receptor mRNAs in the two cell types. The light cell fraction contains larger transcripts of LH/hCG receptor but the heavier Leydig cells contain shorter transcripts. The observations raises the intriguing possibility that shorter rather than larger LH/hCG receptor transcripts are responsible for the induction of a biologically functional, G-protein coupled, LH/hCG receptor in Leydig cells.
引用
收藏
页码:2485 / 2487
页数:3
相关论文
共 19 条
[1]   ON THE STRUCTURE OF THE LUTEINIZING-HORMONE CHORIONIC-GONADOTROPIN RECEPTOR [J].
ASCOLI, M ;
SEGALOFF, DL .
ENDOCRINE REVIEWS, 1989, 10 (01) :27-44
[2]  
BHALLA VK, 1987, J BIOL CHEM, V262, P5322
[3]  
BHALLA VK, 1987, J BIOL CHEM, V262, P5313
[4]  
BHALLA VK, 1978, INT J ANDROL S, V2, P354
[5]   GONADOTROPIN RECEPTOR OCCUPANCY AND STIMULATION OF CAMP AND TESTOSTERONE PRODUCTION BY PURIFIED LEYDIG-CELLS - CRITICAL DEPENDENCE ON CELL CONCENTRATION [J].
BROWNE, ES ;
FLASCH, MV ;
SOHAL, GS ;
BHALLA, VK .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1990, 70 (01) :49-63
[6]  
BROWNE ES, 1990, J ANDROL, V11, P379
[7]   GONADOTROPIN STIMULATION OF CYCLIC ADENOSINE-MONOPHOSPHATE AND TESTOSTERONE PRODUCTION WITHOUT DETECTABLE HIGH-AFFINITY BINDING-SITES IN PURIFIED LEYDIG-CELLS FROM RAT TESTIS [J].
BROWNE, ES ;
BHALLA, VK .
STEROIDS, 1991, 56 (02) :83-90
[8]   GONADAL RECEPTORS .1. EVIDENCE FOR IRREVERSIBILITY IN THE BINDING OF HUMAN CHORIONIC-GONADOTROPIN AND HUMAN LUTEINIZING-HORMONE [J].
CHEN, CJH ;
LINDEMAN, JG ;
TROWBRIDGE, CG ;
BHALLA, VK .
BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 584 (03) :407-435
[9]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[10]   STRUCTURE OF THE LUTEINIZING-HORMONE RECEPTOR GENE AND MULTIPLE EXONS OF THE CODING SEQUENCE [J].
KOO, YB ;
JI, IH ;
SLAUGHTER, RG ;
JI, TH .
ENDOCRINOLOGY, 1991, 128 (05) :2297-2308