ENZYMATIC PRODUCTION OF D-BETA-ACETYLTHIOISOBUTYRIC ACID FROM METHYL DL-BETA-ACETYLTHIOISOBUTYRATE .4. CLONING AND EXPRESSION OF PSEUDOMONAS-PUTIDA ESTERASE GENE IN ESCHERICHIA-COLI AND ITS USE IN ENZYMATIC PRODUCTION OF D-BETA-ACETYLTHIOISOBUTYRIC ACID

被引：17

作者：

OZAKI, E ^{[1
]}

SAKIMAE, A ^{[1
]}

NUMAZAWA, R ^{[1
]}

机构：

[1] MITSUBISHI RAYON CO LTD, CTR PROD DEV, HIGASHI KU, NAGOYA, AICHI 461, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 09期

关键词：

D O I：

10.1271/bbb.58.1745

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The esterase catalyzes the stereoselective hydrolysis of methyl DL-beta-acetylthioisobutyrate (DL-ester) to give D-beta-acetylthioisobutyric acid (DAT). To use the enzyme for DAT production, the esterase gene of Pseudomonas putida was cloned and expressed in E. coli. The recombinant E. coli containing the esterase gene produced a large amount of the enzyme in an active form. This strain could be used for the asymmetric synthesis of DAT.

引用

页码：1745 / 1746

页数：2

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