ISOZYMES FROM CONCHOCELIS OF PORPHYRA SPECIES

The isozyme profiles of Porphyra were examined by means of polyacrylamide gel electrophoresis to establish a method for distinguishing Porphyra species. The conchocelis of Porphyra used in this experiment included P. tenera (Minomi), P. tenera (Matsukawa), P.yezoensis f.narawaensis, P.yezoensis (Saga10), P.yezoensis (Green), and P.seriata (Shimabara). Among the detected enzymes, the electrophoretic profiles of GOT, GDH, PGM, G6PD, ADH, and AK isozymes varied. In particular, the GOT profile distinguished P. tenera, P.yezoensis, and P.seriata, which was the wild type, whereas the AK profile differed in all six species. In addition, the GDH, PGM and ADH profiles distinguished all six species, when combined with those of GOT. In conclusion, the GOT, AK, GDH, PGM and ADH profiles obtained by polyacrylamide gel electrophoresis using conchocelis are thought to be useful for identifying of Porphyra species.