CHARACTERIZATION OF COCHLEAR OUTER HAIR CELL TURGOR

被引:38
作者
CHERTOFF, ME
BROWNELL, WE
机构
[1] JOHNS HOPKINS UNIV,SCH MED,DEPT OTOLARYNGOL HEAD & NECK SURG,BALTIMORE,MD 21205
[2] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI,BALTIMORE,MD 21205
[3] JOHNS HOPKINS UNIV,SCH MED,CTR HEARING SCI,BALTIMORE,MD 21205
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1994年 / 266卷 / 02期
关键词
HYDRAULIC SKELETON; OSMOTIC PRESSURE; MORPHOMETRICS;
D O I
10.1152/ajpcell.1994.266.2.C467
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The cochlear outer hair cell (OHC) is a cylindrical cell with structural features suggestive of a hydraulic skeleton, i.e., an elastic shell with a positive internal pressure. This study characterizes the role of the OHC elevated cytoplasmic pressure in maintaining the cell shape. Intracellular pressure of OHCs from guinea pig is estimated by measuring changes in cell morphology in response to increasing or decreasing osmolarity. Cells collapse when subjected to a continuous increase in osmolarity. Collapse occurs at an average of 8 mosM above the standard medium, suggesting that normal cells have an effective intracellular pressure of 128 mmHg. Fewer cells collapse when exposed to slow rates of osmolarity increase than cells exposed to fast rates of osmolarity increase, although the final change in osmolarity in the perfusion chamber is similar. Furthermore, cells undergo a slow, spontaneous increase in volume on exposure to either no osmolarity change or slow rates of osmolarity increase, suggesting that the cell's internal osmolarity increases in vitro. After volume reduction or elevation, cells do not return to their initial volume.
引用
收藏
页码:C467 / C479
页数:13
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