HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF POLYAMINES IN SELECTED VEGETABLES WITH POSTCOLUMN FLUOROMETRIC DERIVATIZATION

被引：21

作者：

OHTA, H

TAKEDA, Y

YOZA, KI

NOGATA, Y

机构：

[1] Chugoku National Agricultural Experiment Station, Ministry of Agriculture, Fukuyama City, Hiroshima, 721, Forestry and Fisheries

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 628卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)80003-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid high-performance liquid chromatographic analysis for the simultaneous separation of five naturally occuring polyamines (agmatine, putrescine, cadaverine, spermidine and spermine) is described. Postcolumn derivatization with o-phthalaldehyde is used. The separation systems consisted of a strong cation-exchange column, an elution buffer consisting of 1 M sodium citrate (pH 5.4), a mixing coil for the chemical reaction and a spectrofluorimetric detector. The derivatized fluorescent compounds were detected at 340 nm (excitation) and 455 nm (emission). The recoveries of the polyamines were 94.5-107.0% with a standard deviation of 0.83-7.65%.

引用

页码：199 / 204

页数：6

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