A CHIMERIC ACTIN CARRYING N-TERMINAL PORTION OF TETRAHYMENA ACTIN DOES NOT BIND TO DNASE-I

被引：7

作者：

HIRONO, M

SUTOH, K

WATANABE, Y

OHNO, T

机构：

[1] UNIV TSUKUBA,INST BIOL SCI,TSUKUBA,IBARAKI 305,JAPAN

[2] UNIV TOKYO,COLL INTERNAL MED 1,DEPT PURE & APPL SCI,TOKYO 153,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)80054-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A chimeric actin gene was constructed from Tetrahymena actin sequence corresponding to residues 1-83 and Dictyostelium actin sequence corresponding to residues 84-375, and the gene was expressed in Dictyostelium cells. Using DNase I-affinity column, we revealed that the product of the chimeric actin gene was not retained in the column whereas intrinsic actin was retained. In conjunction with our previous data that Tetrahymena actin does not interact with DNase I. © 1992 Academic Press, Inc.

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页码：1511 / 1516

页数：6

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