ISOLATION AND CHARACTERIZATION OF CLONES FOR THE RAT HEPATIC LIPASE GENE UPSTREAM REGULATORY REGION

被引：23

作者：

SENSEL, MG

LEGRANDLORANS, A

WANG, M

BENSADOUN, A

机构：

[1] CORNELL UNIV,DIV NUTR,321 SAVAGE HALL,ITHACA,NY 14853

[2] CORNELL UNIV,DEPT BIOL SCI,ITHACA,NY 14853

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1048卷 / 2-3期

关键词：

Cloning; Gene expression; Lipase; Liver; Regulatory sequence;

D O I：

10.1016/0167-4781(90)90071-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genomic clones for 2287 nucleotides of the 5′ flanking region, 135 nucleotides of the first exon, and 283 nucleotides of the first intron of the hepatic lipase gene were characterized. The predominant start site for transcription was identified by primer extension and S1 nuclease analyses to be 50 bases upstream of the ATG initiation codon. Based on the location of the major transcription start site, the functional TATA box is located 29 nucleotides upstream. Putative response elements for AP-2, cAMP, OCT-1, C/EBP, estrogen, glucocorticoids, sterols and thyroid hormone were located in this gene. Also a putative liver-specific element for apolipoproteins, C3P, was identified. © 1990.

引用

页码：297 / 302

页数：6

共 23 条

[1] EFFECT OF ESTROGEN ON POST-HEPARIN LIPOLYTIC-ACTIVITY - SELECTIVE DECLINE IN HEPATIC TRIGLYCERIDE LIPASE [J].