PROCESSING OF THE PLASMODIUM-FALCIPARUM MAJOR MEROZOITE SURFACE PROTEIN-1 - IDENTIFICATION OF A 33-KILODALTON SECONDARY PROCESSING PRODUCT WHICH IS SHED PRIOR TO ERYTHROCYTE INVASION

被引:133
作者
BLACKMAN, MJ
WHITTLE, H
HOLDER, AA
机构
[1] NATL INST MED RES,DIV PARASITOL,MILL HILL,LONDON NW7 1AA,ENGLAND
[2] MRC LABS,FAJARA,SENEGAMBIA
基金
英国医学研究理事会;
关键词
MALARIA; PLASMODIUM-FALCIPARUM; MEROZOITE SURFACE PROTEIN-1;
D O I
10.1016/0166-6851(91)90128-S
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have previously shown that only a single 19-kDa fragment of the Plasmodium falciparum major merozoite surface protein (MSP1) is carried with an invading merozoite into the infected red cell. This fragment (MSP1(19)) is derived from the C-terminal, membrane-bound end of a major product, MSP1(42), of the primary stage of MSP1 proteolytic processing. Using a monoclonal antibody mapped to an epitope within the N-terminal region Of MSP1(42), we have shown that a soluble 33-kDa polypeptide (MSP1(33)) Corresponding to the N-terminal region of MSP1(42) is shed into culture supernatants during merozoite release and erythrocyte invasion. These observations provide further evidence that the secondary processing Of MSP1(42) involves a highly site-specific proteolytic activity.
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页码:35 / 44
页数:10
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