EXPRESSION IN ESCHERICHIA-COLI AND PURIFICATION OF A CHIMERIC P22-NS3 RECOMBINANT ANTIGEN OF HEPATITIS-C VIRUS (HCV)

被引：4

作者：

OSBORNE, S

CECCONATO, E

GRIVA, S

GARETTO, F

CALOGERO, R

ROSA, C

BONELLI, F

机构：

[1] UNIV NAPOLI FEDERICO 2,DIPARTIMENTO GENET BIOL GEN & MOLEC,VIA MEZZOCANNONE 8,I-80134 NAPLES,ITALY

[2] SORIN BIOMED SPA,DIV R&D DIAGNOST,I-13040 SALUGGIA,ITALY

来源：

FEBS LETTERS | 1993年 / 324卷 / 03期

关键词：

HEPATITIS-C VIRUS; ANTIGEN; FUSION PROTEIN; PURIFICATION; ANTIBODY;

D O I：

10.1016/0014-5793(93)80128-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a starting point for the design of an HCV single all-encompassing antigen for a blood screening assay.

引用

页码：253 / 257

页数：5

共 27 条

[1] DETECTION OF ANTIBODY TO HEPATITIS-C VIRUS IN PROSPECTIVELY FOLLOWED TRANSFUSION RECIPIENTS WITH ACUTE AND CHRONIC NON-A-HEPATITIS, NON-B-HEPATITIS [J].

ALTER, HJ ;

PURCELL, RH ;

SHIH, JW ;

MELPOLDER, JC ;

HOUGHTON, M ;

CHOO, QL ;

KUO, G .

NEW ENGLAND JOURNAL OF MEDICINE, 1989, 321 (22) :1494-1500

[2]

ALTER HJ, CURRENT PERSPECTIVES, P83

[3] TEMPORAL RELATIONSHIPS OF HEPATITIS-C VIRUS-RNA AND ANTIBODY-RESPONSES FOLLOWING EXPERIMENTAL-INFECTION OF CHIMPANZEES [J].

BEACH, MJ ;

MEEKS, EL ;

MIMMS, LT ;

VALLARI, D ;

DUCHARME, L ;

SPELBRING, J ;

TASKAR, S ;

SCHLEICHER, JB ;

KRAWCZYNSKI, K ;

BRADLEY, DW .

JOURNAL OF MEDICAL VIROLOGY, 1992, 36 (03) :226-237

[4]

BOLIVAR F, 1979, METHOD ENZYMOL, V68, P482

[5] SERODIAGNOSIS OF HEPATITIS-C VIRUS (HCV) INFECTION WITH AN HCV CORE PROTEIN MOLECULARLY EXPRESSED BY A RECOMBINANT BACULOVIRUS [J].

CHIBA, J ;

OHBA, H ;

MATSUURA, Y ;

WATANABE, Y ;

KATAYAMA, T ;

KIKUCHI, S ;

SAITO, I ;

MIYAMURA, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (11) :4641-4645

[6] DIAGNOSIS OF HEPATITIS-C VIRUS (HCV) INFECTION USING AN IMMUNODOMINANT CHIMERIC POLYPROTEIN TO CAPTURE CIRCULATING ANTIBODIES - REEVALUATION OF THE ROLE OF HCV IN LIVER-DISEASE [J].

CHIEN, DY ;

CHOO, QL ;

TABRIZI, A ;

KUO, C ;

MCFARLAND, J ;

BERGER, K ;

LEE, C ;

SHUSTER, JR ;

NGUYEN, T ;

MOYER, DL ;

TONG, M ;

FURUTA, S ;

OMATA, M ;

TEGTMEIER, G ;

ALTER, H ;

SCHIFF, E ;

JEFFERS, L ;

HOUGHTON, M ;

KUO, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10011-10015

[7] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME [J].

CHOO, QL ;

KUO, G ;

WEINER, AJ ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :359-362

[8] GENETIC ORGANIZATION AND DIVERSITY OF THE HEPATITIS-C VIRUS [J].

CHOO, QL ;

RICHMAN, KH ;

HAN, JH ;

BERGER, K ;

LEE, C ;

DONG, C ;

GALLEGOS, C ;

COIT, D ;

MEDINASELBY, A ;

BARR, PJ ;

WEINER, AJ ;

BRADLEY, DW ;

KUO, G ;

HOUGHTON, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2451-2455

[9] POLYETHYLENE-GLYCOL ENHANCED PROTEIN REFOLDING [J].

CLELAND, JL ;

BUILDER, SE ;

SWARTZ, JR ;

WINKLER, M ;

CHANG, JY ;

WANG, DIC .

BIO-TECHNOLOGY, 1992, 10 (09) :1013-1019

[10] VERSATILE EXPRESSION VECTORS FOR HIGH-LEVEL SYNTHESIS OF CLONED GENE-PRODUCTS IN ESCHERICHIA-COLI [J].

CROWL, R ;

SEAMANS, C ;

LOMEDICO, P ;

MCANDREW, S .

GENE, 1985, 38 (1-3) :31-38

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