共 32 条
ANTISENSE PHOSPHOROTHIOATE OLIGONUCLEOTIDES - SELECTIVE KILLING OF THE INTRACELLULAR PARASITE LEISHMANIA-AMAZONENSIS
被引:43
作者:

RAMAZEILLES, C
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV BORDEAUX 2,INSERM,U386,MOLEC BIOPHYS LAB,F-33076 BORDEAUX,FRANCE UNIV BORDEAUX 2,INSERM,U386,MOLEC BIOPHYS LAB,F-33076 BORDEAUX,FRANCE

MISHRA, RK
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV BORDEAUX 2,INSERM,U386,MOLEC BIOPHYS LAB,F-33076 BORDEAUX,FRANCE UNIV BORDEAUX 2,INSERM,U386,MOLEC BIOPHYS LAB,F-33076 BORDEAUX,FRANCE

MOREAU, S
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV BORDEAUX 2,INSERM,U386,MOLEC BIOPHYS LAB,F-33076 BORDEAUX,FRANCE UNIV BORDEAUX 2,INSERM,U386,MOLEC BIOPHYS LAB,F-33076 BORDEAUX,FRANCE

PASCOLO, E
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV BORDEAUX 2,INSERM,U386,MOLEC BIOPHYS LAB,F-33076 BORDEAUX,FRANCE UNIV BORDEAUX 2,INSERM,U386,MOLEC BIOPHYS LAB,F-33076 BORDEAUX,FRANCE

TOULME, JJ
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV BORDEAUX 2,INSERM,U386,MOLEC BIOPHYS LAB,F-33076 BORDEAUX,FRANCE UNIV BORDEAUX 2,INSERM,U386,MOLEC BIOPHYS LAB,F-33076 BORDEAUX,FRANCE
机构:
[1] UNIV BORDEAUX 2,INSERM,U386,MOLEC BIOPHYS LAB,F-33076 BORDEAUX,FRANCE
来源:
关键词:
PROTOZOAN PARASITE;
LOW DENSITY LIPOPROTEINS;
HETERODUPLEX STABILITY;
RNASE H;
D O I:
10.1073/pnas.91.17.7859
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
We targeted the mini-exon sequence, present at the 5' end of every mRNA of the protozoan parasite Leishmania amazonensis, by phosphorothioate oligonucleotides. A complementary 16-mer (16PS) was able to kill amastigotes-the intracellular stage of the parasite-in murine macrophages in culture. After 24 hr of incubation with 10 mu M 16PS, about 30% infected macrophages were cured. The oligomer 16PS acted through antisense hybridization in a sequence-dependent way; no effect on parasites was observed with noncomplementary phosphorothioate oligonucleotides. The antisense oligonucleotide 16PS was a selective killer of the protozoans without any detrimental effect to the host macrophage. Using 16PS linked to a palmitate chain, which enabled it to complex with low density lipoproteins, improved the leishmanicidal efficiency on intracellular amastigotes, probably due to increased endocytosis. Phosphorothioate oligonucleotides complementary to the intron part of the mini-exon pre-RNA were also effective, suggesting that antisense oligomers could prevent trans-splicing in these parasites.
引用
收藏
页码:7859 / 7863
页数:5
相关论文
共 32 条
- [1] COMPARATIVE ACTIVITY OF ALPHA-ANOMERIC AND BETA-ANOMERIC OLIGONUCLEOTIDES ON RABBIT BETA-GLOBIN SYNTHESIS - INHIBITORY EFFECT OF CAP TARGETED ALPHA-OLIGONUCLEOTIDES[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (01) : 311 - 318BERTRAND, JR论文数: 0 引用数: 0 h-index: 0机构: UNIV MONTPELLIER 2,CNRS,UA 488,F-34060 MONTPELLIER,FRANCE UNIV MONTPELLIER 2,CNRS,UA 488,F-34060 MONTPELLIER,FRANCEIMBACH, JL论文数: 0 引用数: 0 h-index: 0机构: UNIV MONTPELLIER 2,CNRS,UA 488,F-34060 MONTPELLIER,FRANCE UNIV MONTPELLIER 2,CNRS,UA 488,F-34060 MONTPELLIER,FRANCEPAOLETTI, C论文数: 0 引用数: 0 h-index: 0机构: UNIV MONTPELLIER 2,CNRS,UA 488,F-34060 MONTPELLIER,FRANCE UNIV MONTPELLIER 2,CNRS,UA 488,F-34060 MONTPELLIER,FRANCEMALVY, C论文数: 0 引用数: 0 h-index: 0机构: UNIV MONTPELLIER 2,CNRS,UA 488,F-34060 MONTPELLIER,FRANCE UNIV MONTPELLIER 2,CNRS,UA 488,F-34060 MONTPELLIER,FRANCE
- [2] INHIBITION OF TRANSLATION INITIATION BY ANTISENSE OLIGONUCLEOTIDES VIA AN RNASE-H INDEPENDENT MECHANISM[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (05) : 1113 - 1119BOIZIAU, C论文数: 0 引用数: 0 h-index: 0机构: MUSEUM NATL HIST NAT,BIOPHYS LAB,F-75231 PARIS 05,FRANCEKURFURST, R论文数: 0 引用数: 0 h-index: 0机构: MUSEUM NATL HIST NAT,BIOPHYS LAB,F-75231 PARIS 05,FRANCECAZENAVE, C论文数: 0 引用数: 0 h-index: 0机构: MUSEUM NATL HIST NAT,BIOPHYS LAB,F-75231 PARIS 05,FRANCEROIG, V论文数: 0 引用数: 0 h-index: 0机构: MUSEUM NATL HIST NAT,BIOPHYS LAB,F-75231 PARIS 05,FRANCETHUONG, NT论文数: 0 引用数: 0 h-index: 0机构: MUSEUM NATL HIST NAT,BIOPHYS LAB,F-75231 PARIS 05,FRANCETOULME, JJ论文数: 0 引用数: 0 h-index: 0机构: MUSEUM NATL HIST NAT,BIOPHYS LAB,F-75231 PARIS 05,FRANCE
- [3] DISCONTINUOUS TRANSCRIPTION AND ANTIGENIC VARIATION IN TRYPANOSOMES[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 : 701 - 732BORST, P论文数: 0 引用数: 0 h-index: 0
- [4] TRANS SPLICING INVOLVES A NOVEL FORM OF SMALL NUCLEAR RIBONUCLEOPROTEIN-PARTICLES[J]. NATURE, 1988, 335 (6190) : 559 - 562BRUZIK, JP论文数: 0 引用数: 0 h-index: 0机构: SYNERGEN INC,BOULDER,CO 80301 SYNERGEN INC,BOULDER,CO 80301VANDOREN, K论文数: 0 引用数: 0 h-index: 0机构: SYNERGEN INC,BOULDER,CO 80301 SYNERGEN INC,BOULDER,CO 80301HIRSH, D论文数: 0 引用数: 0 h-index: 0机构: SYNERGEN INC,BOULDER,CO 80301 SYNERGEN INC,BOULDER,CO 80301STEITZ, JA论文数: 0 引用数: 0 h-index: 0机构: SYNERGEN INC,BOULDER,CO 80301 SYNERGEN INC,BOULDER,CO 80301
- [5] RATE OF DEGRADATION OF [ALPHA]-OLIGODEOXYNUCLEOTIDES AND [BETA]-OLIGODEOXYNUCLEOTIDES IN XENOPUS OOCYTES - IMPLICATIONS FOR ANTI-MESSENGER STRATEGIES[J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (24) : 10507 - 10521CAZENAVE, C论文数: 0 引用数: 0 h-index: 0机构: CNRS,CTR BIOPHYS MOLEC,F-45071 ORLEANS 02,FRANCE CNRS,CTR BIOPHYS MOLEC,F-45071 ORLEANS 02,FRANCECHEVRIER, M论文数: 0 引用数: 0 h-index: 0机构: CNRS,CTR BIOPHYS MOLEC,F-45071 ORLEANS 02,FRANCE CNRS,CTR BIOPHYS MOLEC,F-45071 ORLEANS 02,FRANCETHUONG, NT论文数: 0 引用数: 0 h-index: 0机构: CNRS,CTR BIOPHYS MOLEC,F-45071 ORLEANS 02,FRANCE CNRS,CTR BIOPHYS MOLEC,F-45071 ORLEANS 02,FRANCEHELENE, C论文数: 0 引用数: 0 h-index: 0机构: CNRS,CTR BIOPHYS MOLEC,F-45071 ORLEANS 02,FRANCE CNRS,CTR BIOPHYS MOLEC,F-45071 ORLEANS 02,FRANCE
- [6] COMPARATIVE INHIBITION OF RABBIT GLOBIN MESSENGER-RNA TRANSLATION BY MODIFIED ANTISENSE OLIGODEOXYNUCLEOTIDES[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (11) : 4255 - 4273CAZENAVE, C论文数: 0 引用数: 0 h-index: 0机构: CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS, FRANCESTEIN, CA论文数: 0 引用数: 0 h-index: 0机构: CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS, FRANCELOREAU, N论文数: 0 引用数: 0 h-index: 0机构: CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS, FRANCETHUONG, NT论文数: 0 引用数: 0 h-index: 0机构: CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS, FRANCENECKERS, LM论文数: 0 引用数: 0 h-index: 0机构: CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS, FRANCESUBASINGHE, C论文数: 0 引用数: 0 h-index: 0机构: CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS, FRANCEHELENE, C论文数: 0 引用数: 0 h-index: 0机构: CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS, FRANCECOHEN, JS论文数: 0 引用数: 0 h-index: 0机构: CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS, FRANCETOULME, JJ论文数: 0 引用数: 0 h-index: 0机构: CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS, FRANCE
- [7] THE COMMON 5' TERMINAL SEQUENCE ON TRYPANOSOME MESSENGER-RNAS - A TARGET FOR ANTI-MESSENGER OLIGODEOXYNUCLEOTIDES[J]. NUCLEIC ACIDS RESEARCH, 1986, 14 (14) : 5605 - 5614CORNELISSEN, AWCA论文数: 0 引用数: 0 h-index: 0机构: MUSEUM NATL HIST NAT,CNRS,ERA 481,INSERM,U201,BIOPHYS LAB,F-75231 PARIS 05,FRANCE MUSEUM NATL HIST NAT,CNRS,ERA 481,INSERM,U201,BIOPHYS LAB,F-75231 PARIS 05,FRANCEVERSPIEREN, MP论文数: 0 引用数: 0 h-index: 0机构: MUSEUM NATL HIST NAT,CNRS,ERA 481,INSERM,U201,BIOPHYS LAB,F-75231 PARIS 05,FRANCE MUSEUM NATL HIST NAT,CNRS,ERA 481,INSERM,U201,BIOPHYS LAB,F-75231 PARIS 05,FRANCETOULME, JJ论文数: 0 引用数: 0 h-index: 0机构: MUSEUM NATL HIST NAT,CNRS,ERA 481,INSERM,U201,BIOPHYS LAB,F-75231 PARIS 05,FRANCE MUSEUM NATL HIST NAT,CNRS,ERA 481,INSERM,U201,BIOPHYS LAB,F-75231 PARIS 05,FRANCESWINKELS, BW论文数: 0 引用数: 0 h-index: 0机构: MUSEUM NATL HIST NAT,CNRS,ERA 481,INSERM,U201,BIOPHYS LAB,F-75231 PARIS 05,FRANCE MUSEUM NATL HIST NAT,CNRS,ERA 481,INSERM,U201,BIOPHYS LAB,F-75231 PARIS 05,FRANCEBORST, P论文数: 0 引用数: 0 h-index: 0机构: MUSEUM NATL HIST NAT,CNRS,ERA 481,INSERM,U201,BIOPHYS LAB,F-75231 PARIS 05,FRANCE MUSEUM NATL HIST NAT,CNRS,ERA 481,INSERM,U201,BIOPHYS LAB,F-75231 PARIS 05,FRANCE
- [8] ASSOCIATION OF ANTISENSE OLIGONUCLEOTIDES WITH LIPOPROTEINS PROLONGS THE PLASMA HALF-LIFE AND MODIFIES THE TISSUE DISTRIBUTION[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (17) : 4695 - 4700DESMIDT, PC论文数: 0 引用数: 0 h-index: 0机构: LEIDEN UNIV,SYLVIUS LAB,CTR BIOPHARMACEUT SCI,DIV BIOPHARMACEUT,POB 9503,2300 RA LEIDEN,NETHERLANDSLEDOAN, T论文数: 0 引用数: 0 h-index: 0机构: LEIDEN UNIV,SYLVIUS LAB,CTR BIOPHARMACEUT SCI,DIV BIOPHARMACEUT,POB 9503,2300 RA LEIDEN,NETHERLANDSDEFALCO, S论文数: 0 引用数: 0 h-index: 0机构: LEIDEN UNIV,SYLVIUS LAB,CTR BIOPHARMACEUT SCI,DIV BIOPHARMACEUT,POB 9503,2300 RA LEIDEN,NETHERLANDSVANBERKEL, TJC论文数: 0 引用数: 0 h-index: 0机构: LEIDEN UNIV,SYLVIUS LAB,CTR BIOPHARMACEUT SCI,DIV BIOPHARMACEUT,POB 9503,2300 RA LEIDEN,NETHERLANDS
- [9] ALPHA-DNA-VI - COMPARATIVE-STUDY OF ALPHA-ANOMERIC AND BETA-ANOMERIC OLIGODEOXYRIBONUCLEOTIDES IN HYBRIDIZATION TO MESSENGER-RNA AND IN CELL FREE TRANSLATION INHIBITION[J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (24) : 10419 - 10436GAGNOR, C论文数: 0 引用数: 0 h-index: 0机构: INST GUSTAVE ROUSSY, CNRS, LAB 147, 53 RUE CAMILLE DESMOULINS, F-94800 VILLEJUIF, FRANCEBERTRAND, JR论文数: 0 引用数: 0 h-index: 0机构: INST GUSTAVE ROUSSY, CNRS, LAB 147, 53 RUE CAMILLE DESMOULINS, F-94800 VILLEJUIF, FRANCETHENET, S论文数: 0 引用数: 0 h-index: 0机构: INST GUSTAVE ROUSSY, CNRS, LAB 147, 53 RUE CAMILLE DESMOULINS, F-94800 VILLEJUIF, FRANCELEMAITRE, M论文数: 0 引用数: 0 h-index: 0机构: INST GUSTAVE ROUSSY, CNRS, LAB 147, 53 RUE CAMILLE DESMOULINS, F-94800 VILLEJUIF, FRANCEMORVAN, F论文数: 0 引用数: 0 h-index: 0机构: INST GUSTAVE ROUSSY, CNRS, LAB 147, 53 RUE CAMILLE DESMOULINS, F-94800 VILLEJUIF, FRANCERAYNER, B论文数: 0 引用数: 0 h-index: 0机构: INST GUSTAVE ROUSSY, CNRS, LAB 147, 53 RUE CAMILLE DESMOULINS, F-94800 VILLEJUIF, FRANCEMALVY, C论文数: 0 引用数: 0 h-index: 0机构: INST GUSTAVE ROUSSY, CNRS, LAB 147, 53 RUE CAMILLE DESMOULINS, F-94800 VILLEJUIF, FRANCELEBLEU, B论文数: 0 引用数: 0 h-index: 0机构: INST GUSTAVE ROUSSY, CNRS, LAB 147, 53 RUE CAMILLE DESMOULINS, F-94800 VILLEJUIF, FRANCEIMBACH, JL论文数: 0 引用数: 0 h-index: 0机构: INST GUSTAVE ROUSSY, CNRS, LAB 147, 53 RUE CAMILLE DESMOULINS, F-94800 VILLEJUIF, FRANCEPAOLETTI, C论文数: 0 引用数: 0 h-index: 0机构: INST GUSTAVE ROUSSY, CNRS, LAB 147, 53 RUE CAMILLE DESMOULINS, F-94800 VILLEJUIF, FRANCE
- [10] SPECIFIC REGULATION OF GENE-EXPRESSION BY ANTISENSE, SENSE AND ANTIGENE NUCLEIC-ACIDS[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1049 (02) : 99 - 125HELENE, C论文数: 0 引用数: 0 h-index: 0机构: Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U201-CNRS UA.481, ParisTOULME, JJ论文数: 0 引用数: 0 h-index: 0机构: Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U201-CNRS UA.481, Paris