SYNTHESIS OF 1-DEOXY-D-RIBOFURANOSE PHOSPHORAMIDITE AND THE INCORPORATION OF ABASIC NUCLEOTIDES IN STEM-LOOP-II OF A HAMMERHEAD RIBOZYME

被引：31

作者：

BEIGELMAN, L ^{[1
]}

KARPEISKY, A ^{[1
]}

USMAN, N ^{[1
]}

机构：

[1] RIBOZYME PHARMACEUT INC,DEPT CHEM & BIOCHEM,2950 WILDERNESS PL,BOULDER,CO 80301

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1994年 / 4卷 / 14期

关键词：

D O I：

10.1016/S0960-894X(00)80367-4

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The synthesis of 1-deoxy-D-ribofuranose-3-(2-cyanoethyl N,N-diisopropylphosphoramidite) 9 from D-ribose and its incorporation in a hammerhead ribozyme is described. Ribozymes containing 4, 6 or 8 abasic nucleotides, H, in Loop and Stem II have wild-type catalytic activity.

引用

页码：1715 / 1720

页数：6

共 25 条

[1] SYNTHESIS OF 1,2-TRANS-RELATED 1-THIOGLYCOSIDE ESTERS
FERRIER, RJ
FURNEAUX, RH
[J]. CARBOHYDRATE RESEARCH, 1976, 52 (DEC) : 63 - 68
[2] IMPORTANCE OF SPECIFIC PURINE AMINO AND HYDROXYL-GROUPS FOR EFFICIENT CLEAVAGE BY A HAMMERHEAD RIBOZYME
FU, DJ
MCLAUGHLIN, LW
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (09) : 3985 - 3989
[3] IMPORTANCE OF SPECIFIC ADENOSINE N(7)-NITROGENS FOR EFFICIENT CLEAVAGE BY A HAMMERHEAD RIBOZYME - A MODEL FOR MAGNESIUM BINDING
FU, DJ
MCLAUGHLIN, LW
[J]. BIOCHEMISTRY, 1992, 31 (45) : 10941 - 10949
[4] FU DJ, 1993, J AM CHEM SOC, V115, P8483
[5] SYNTHESIS OF 2-DEOXY SUGARS
GIESE, B
GRONINGER, KS
WITZEL, T
KORTH, HG
SUSTMANN, R
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1987, 26 (03): : 233 - 234
[6] ONE ELECTRON C-C BOND FORMING REACTIONS VIA ALLYLSTANNANES - SCOPE AND LIMITATIONS
KECK, GE
ENHOLM, EJ
YATES, JB
WILEY, MR
[J]. TETRAHEDRON, 1985, 41 (19) : 4079 - 4094
[7] BEHAVIOR OF SOME ALDOSES WITH 2,2-DIALKOXYPROPANE-N,N-DIMETHYLFORMAMIDE-PARA-TOLUENE-SULFONIC ACID .5. ACETONATION OF SOME PENTOSES WITH 2,2-DIMETHOXYPROPANE-N,N-DIMETHYLFORMAMIDE-PARA-TOLUENESULFONIC ACID
KISO, M
HASEGAWA, A
[J]. CARBOHYDRATE RESEARCH, 1976, 52 (DEC) : 95 - 101
[8] SYNTHESIS AND BIOPHYSICAL STUDIES OF SHORT OLIGODEOXYNUCLEOTIDES WITH NOVEL MODIFICATIONS - A POSSIBLE APPROACH TO THE PROBLEM OF MIXED BASE OLIGODEOXYNUCLEOTIDE SYNTHESIS
MILLICAN, TA
MOCK, GA
CHAUNCEY, MA
PATEL, TP
EATON, MAW
GUNNING, J
CUTBUSH, SD
NEIDLE, S
MANN, J
[J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (19) : 7435 - 7453
[9] OHRUI H, 1973, TETRAHEDRON LETT, P1951
[10] NUCLEASE RESISTANT RIBOZYMES WITH HIGH CATALYTIC ACTIVITY
PAOLELLA, G
SPROAT, BS
LAMOND, AI
[J]. EMBO JOURNAL, 1992, 11 (05) : 1913 - 1919

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