MOLECULAR ACTION OF THE TYPE-1 RIBOSOME-INACTIVATING PROTEIN SAPORIN-5 ON VICIA-SATIVA RIBOSOMES

被引：28

作者：

IGLESIAS, R

ARIAS, FJ

ROJO, MA

ESCARMIS, C

FERRERAS, JM

GIRBES, T

机构：

[1] UNIV VALLADOLID,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC,E-47005 VALLADOLID,SPAIN

[2] UAM,CSIC,CTR BIOL MOLEC,MADRID,SPAIN

来源：

FEBS LETTERS | 1993年 / 325卷 / 03期

关键词：

RIBOSOME-INACTIVATING PROTEIN; SAPORIN-5; VICIA-SATIVA; PROTEIN SYNTHESIS;

D O I：

10.1016/0014-5793(93)81091-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The type 1 ribosome-inactivating protein (RIP) saporin 5 isolated from seeds of Saponaria officinalis L. strongly inhibited translation carried out by Vicia sativa L. purified ribosomes. The toxin multidepurinated V. sativa rRNA, which upon treatment with acid aniline releases several RNA fragments including an RNA fragment of approximately 370 nucleotides the 5'-end sequence of which was 5'-GAGGAACG-3'.

引用

页码：291 / 294

页数：4

共 24 条

[1]

ARIAS FJ, 1992, PLANTA, V186, P532, DOI 10.1007/BF00198033

[2] PREPARATION AND OPTIMIZATION OF A CELL-FREE TRANSLATION SYSTEM FROM VICIA-SATIVA GERM LACKING RIBOSOME-INACTIVATING PROTEIN-ACTIVITY [J].