A NOVEL ISOENZYME OF ASCORBATE PEROXIDASE LOCALIZED ON GLYOXYSOMAL AND LEAF PEROXISOMAL MEMBRANES IN PUMPKIN

被引：152

作者：

YAMAGUCHI, K ^{[1
]}

MORI, H ^{[1
]}

NISHIMURA, M ^{[1
]}

机构：

[1] NATL INST BASIC BIOL,DEPT CELL BIOL,OKAZAKI,AICHI 444,JAPAN

来源：

PLANT AND CELL PHYSIOLOGY | 1995年 / 36卷 / 06期

关键词：

ASCORBATE PEROXIDASE; GLYOXYSOME; HYDROGEN PEROXIDE; LEAF PEROXISOME; PHOTORESPIRATION;

D O I：

10.1093/oxfordjournals.pcp.a078862

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A novel isoenzyme of ascorbate peroxidase with molecular mass of 31 kDa was found to be localized on membranes of microbodies. Intact microbodies had no latent ascorbate peroxidase activity, an indication that the active site of the ascorbate peroxidase was exposed to the cytosol and the peroxidase would scavenge hydrogen peroxide leaked from microbodies.

引用

页码：1157 / 1162

页数：6

共 28 条

[1] Amon D.I., Copper enzymes in chloroplasts. Polyphenol oxidase in Beta vulgaris, Plant Physiol, 24, pp. 1-15, (1949)
[2] Arrigoni O., Dipierro S., Borracdno O., Ascorbate free radical reductase, a key enzyme of the ascorbic acid system, FEBS Lett, 125, pp. 242-244, (1981)
[3] Chance B., Sies H., Boveris A., Hydroperoxide metabolism in mammalian organs, Physiol. Rev, 59, pp. 527-605, (1979)
[4] Chen G., Asada K., Ascorbate peroxidase in tea leaves: Occurrence of two isozymes and the differences in their enzymatic and molecular properties, Plant Cell Physiol, 30, pp. 987-998, (1989)
[5] Chen Z., Silva H., Klessig D.F., Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid, Science, 262, pp. 1883-1886, (1993)
[6] Davison A.J., Kettle A.J., Fatur D.J., Mechanism of the inhibition of catalase by ascorbate, J. Biol. Chem, 261, pp. 1193-1200, (1986)
[7] De Duve C., Baudhuin P., Peroxisomes (Microbodies and related particles), Physiol. Rev, 46, pp. 323-357, (1966)
[8] Drumm-Herrel H., Gerhausser U., Mohr H., Differential regulation by phytochrome of the appearance of plastidic and cytoplasmic isoforms of glutathione reductase in mustard (.Sinapis alba L.) cotyledons, Planta, 178, pp. 103-109, (1989)
[9] Edwards E.A., Stephen R., Philip M.M., Subcellular distribution of multiple forms of glutathione reductase in leaves of pea (Pisum sativum L.), Planta, 180, pp. 278-284, (1990)
[10] Feierabend J., Developmental studies on microbodies in wheat leaves. On the photocontrol of microbody development, Planta, 123, pp. 63-77, (1975)

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