CHEMICAL-STRUCTURE OF THE CARBOHYDRATE BACKBONE OF VIBRIO-PARAHAEMOLYTICUS SEROTYPE-012 LIPOPOLYSACCHARIDE

The chemical structure of the saccharide portion of Vibrio parahaemolyticus serotype 012 lipopolysaccharide was studied. Using chemical degradation and modification, as well as methylation analysis in combination with GLC-MS, laser-desorption mass spectrometry and H-1-NMR and C-13-NMR spectroscopy, the carbohydrate backbone of the lipopolysaccharide was characterized as a branched decasaccharide with the following structure: [GRAPHICS] In the native lipopolysaccharide two additional phosphate groups are present and 3-deoxy-D-threo-hexulosonic acid and D-galacturonic acid are bound via acid-labile linkages.