AUTOANTIBODIES TO THE RO/SSA AUTOANTIGEN ARE CONFORMATION DEPENDENT-II - ANTIBODIES TO THE DENATURED FORM OF 52-KD RO/SSA ARE A CROSS REACTING SUBSET OF ANTIBODIES TO THE NATIVE 60-KD RO/SSA MOLECULE

被引：57

作者：

ITOH, Y ^{[1
]}

ITOH, K ^{[1
]}

FRANK, MB ^{[1
]}

REICHLIN, M ^{[1
]}

机构：

[1] UNIV OKLAHOMA HLTH SCI CTR,OKLAHOMA MED RES FDN,COLL MED,DEPT MED,OKLAHOMA CITY,OK 73104

来源：

AUTOIMMUNITY | 1992年 / 14卷 / 02期

关键词：

AUTOANTIBODY; RIBONUCLEOPROTEIN; HETEROGENEITY; SYSTEMIC LUPUS ERYTHEMATOSUS; PRIMARY SJOGRENS SYNDROME;

D O I：

10.3109/08916939209083125

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The immune response to the Ro/SSA particles is conformation dependent. In sera with only anti-Ro/SSA precipitins, the autoantibodies to the 60 kD Ro/SSA are largely to the native 60 kD Ro/SSA while autoantibodies to the 52 kD Ro/SSA particle when present are exclusively to the denatured 52 kD Ro/SSA particle. Antibodies eluted from a recombinant 52 kD Ro/SSA fusion protein reacted in a sandwich ELISA which only measures antibody to native 60 kD Ro/SSA antigens and this reaction is largely inhibited by native homogeneous 60 kD Ro/SSA. In addition, antibody binding to the 52 kD Ro/SSA antigen in Western blot is also strongly inhibited by native 60 kD Ro/SSA. These experiment strongly suggest that reactivity of denatured 52 kD Ro/SSA antigen represents a cross reaction with autoantibodies directed to the native 60 kD Ro/SSA antigen. As a corollary of these experiments. data are presented that suggest the hY-RNAs are not associated with the 52 kD Ro/SSA protein but only with the 60 kD Ro/SSA protein. These data are consistent with the hypothesis that the autoanti-Ro/SSA response is driven by native 60 kD Ro/SSA and the immune response to denatured 52 kD Ro/SSA is largely a cross-reactive subset of the immune response to native 60 kD Ro/SSA.

引用

页码：89 / 95

页数：7

共 14 条

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