EXPRESSION OF A PEA DISEASE RESISTANCE RESPONSE GENE IN THE POTATO CULTIVAR SHEPODY

被引：30

作者：

CHANG, MM ^{[1
]}

CHIANG, CC ^{[1
]}

MARTIN, MW ^{[1
]}

HADWIGER, LA ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV,USDA ARS,CTR IRRIGATED AGR RES & EXTENS,PROSSER,WA 99350

来源：

AMERICAN POTATO JOURNAL | 1993年 / 70卷 / 09期

关键词：

TRANSFORMATION; GENETIC ENGINEERING; TRANSGENIC PLANTS;

D O I：

10.1007/BF02849153

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The coding sequence from the pea disease resistance response gene 49 (DRR49 gene), was transferred into the potato (Solanum tuberosum L.) cultivar Shepody via Agrobacterium co-cultivation. Potato leaf explants were co-cultivated with Agrobacterium tumefaciens harboring a binary vector containing the coding sequence from the DRR49 gene under the control of the 35S promoter from cauliflower mosaic virus (CaMV). Transformed potato plants were selected by their resistance to kanamycin. The insertion of foreign DNAs was detected by polymerase chain reaction (PCR) and Southern blot analysis. The expression of DRR49 coding sequence was confirmed via the detection of the corresponding mRNA in northern blot analysis. Field pathogenicity tests indicated that transgenic plants expressing the pea DRR49 mRNA have higher tuber yields than the control plants when grown in PED (potato early dying) infested soil.

引用

页码：635 / 647

页数：13

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