QUANTIFICATION OF DIMETHINDENE IN PLASMA BY GAS-CHROMATOGRAPHY MASS FRAGMENTOGRAPHY USING AMMONIA CHEMICAL-IONIZATION

被引：4

作者：

KAUERT, G ^{[1
]}

HERRLE, I ^{[1
]}

WERMEILLE, M ^{[1
]}

机构：

[1] ZYMA SA,CH-1260 NYON,SWITZERLAND

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 617卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80506-Y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A gas chromatographic-mass fragmentographic method using ammonia chemical ionization for the determination of dimethindene in human plasma is described. The drug was isolated from plasma by liquid-liquid extraction with hexane-2-methylbutanol. Plasma components were separated on a capillary column coated with chemically bonded methyl silicone. For detection of dimethindene, its quasi-molecular ion (M + H+) was mass fragmentographically monitored after chemical ionization with ammonia as reagent gas. Dimethindene was quantified using methaqualone as the internal standard: the quantification limit in plasma was 0.2 ng/ml, the within-run precision was 8.0% and the inter-run precision 5.6%. The plasma concentration-time profile was established after a single dose of 4 mg of dimethindene with an average maximum concentration of 5.5 ng/ml, detectable up to 48 h post application.

引用

页码：318 / 323

页数：6