APPLICATION OF MONOCLONAL-ANTIBODIES TO QUALITY-CONTROL OF FOOT-AND-MOUTH-DISEASE VACCINES

被引：9

作者：

ALONSO, A

DARSIE, GC

TEIXEIRA, AC

REIS, JL

MESQUITA, JA

机构：

[1] Pan American Foot-and-Mouth Disease Center (PAHO/WHO), 20.001 Rio de Janeiro

来源：

VACCINE | 1994年 / 12卷 / 08期

关键词：

FOOT-AND-MOUTH DISEASE; VACCINES; MONOCLONAL ANTIBODIES; ELISA;

D O I：

10.1016/0264-410X(94)90216-X

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Panels of monoclonal antibodies (mAbs) produced against-foot-and-mouth disease (FMD) virus types O, A and C were selected for cell culture neutralization titre (NT), mouse protection index (MPI), trypsin sensitivity (TSI and avidity to different epitopes. The selected sets were used to assay the antigen concentration and the Jit between FMDV vaccine and challenge strains. It was observed that FMD vaccines protect more than 75% of vaccinated cattle when manufactured with antigens characterized by (1) a high degree of fit with the potency control virus, and (2) mean ELISA 50% titres (T-50) > 28 for O, > 18 for A and > 75 for C types, respectively, using the cor responding mAb set.

引用

页码：682 / 686

页数：5

共 28 条

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