OSTERTAGIA-OSTERTAGI - ISOLATION AND PARTIAL CHARACTERIZATION OF SOMATIC AND METABOLIC ANTIGENS

被引:47
作者
CANALS, A
GASBARRE, LC
机构
[1] USDA ARS,BELTSVILLE AGR RES CTR,INST ANIM PARASITOL,HELMINTH DIS LAB,BELTSVILLE,MD 20705
[2] INST NACL INVEST AGR,MADRID,SPAIN
关键词
NEMATODA; PARASITIC; CATTLE; IMMUNOLOGY; GASTROINTESTINAL; ANTIGENS; OSTERTAGIA-OSTERTAGI;
D O I
10.1016/0020-7519(90)90048-R
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
One metabolic (ES) and two somatic extracts (AS and MS) were prepared from Ostertagia ostertagi. Partial characterization of the three preparations was accomplished by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot techniques. In immunoblots, AS showed a low number of bands that reacted weakly with sera collected from infected calves. MS reacted strongly with the homologous sera, and a sharp group of bands appeared from 12 to 14 kDa, increasing in intensity as the infection progressed. ES showed a group of strongly immunogenic bands in the range of 16-22 kDa. The three preparations were also tested for reactivity with specific anti-Ostertagia antibodies in an enzyme-linked immunosorbant assay (ELISA). The ELISA results showed that all three worm extracts contained antigen epitopes recognized by circulating antibody in sera taken from O. ostertagi-infected calves. The strongest response was seen when antibodies of the IgG1 isotype were reacted with MS and ES. When sera taken from O. radiatum-infected calves were used both somatic extracts showed high levels of cross-reactivity.
引用
收藏
页码:1047 / 1054
页数:8
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