IMMUNO PRECOLUMNS FOR SELECTIVE ONLINE SAMPLE PRETREATMENT OF AFLATOXINS IN MILK PRIOR TO COLUMN LIQUID-CHROMATOGRAPHY

被引:16
作者
FARJAM, A
DEVRIES, R
LINGEMAN, H
BRINKMAN, UAT
机构
[1] Department of Analytical Chemistry, Free University, 1081 HV Amsterdam
关键词
IMMUNOAFFINITY PRECOLUMNS; COLUMN SWITCHING; COLUMN LIQUID CHROMATOGRAPHY; AFLATOXINS; MILK;
D O I
10.1080/03067319108027549
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A column liquid chromatographic system for on-line sample pretreatment and determination of aflatoxin M1 in milk is described. The system consists of an immuno precolumn packed with immobilized monoclonal or polyclonal antibodies, a second precolumn packed with C-18 bonded silica and a C-18 analytical column. Defatted milk is directly loaded on the immuno precolumn. Desorption takes place by eluting the immuno precolumn with methanol-water (70:30, v/v). The eluate is diluted on-line with water with subsequent reconcentration of the analytes on the C-18 bonded silica precolumn and separation on the analytical column followed by fluorescence detection. The applicability of automated immunoaffinity sample preparation with both a polyclonal and a monoclonal immuno precolumn is discussed and aspects such as the interference of milk proteins and the use of an external standard are investigated. Both types of immuno precolumns can be used for at least 20 analyses of milk samples, with relative standard deviations of 5-10% and detection limits of 20 ng/1 for aflatoxin M1 in milk. The linear dynamic range is 20–400 ng/1 of aflatoxin M1 in milk if 2.4-ml milk samples are used. © 1991, Taylor & Francis Group, LLC. All rights reserved.
引用
收藏
页码:175 / 184
页数:10
相关论文
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