PRODUCTION AND CHARACTERIZATION OF ANTI-THEOPHYLLINE MONOCLONAL-ANTIBODIES SUITABLE FOR IMMUNOASSAY

被引：8

作者：

DANILOVA, ND

VASILOV, RG

机构：

[1] Department of Medical Diagnostics, Institute of Biotechnology, Moscow

来源：

IMMUNOLOGY LETTERS | 1991年 / 28卷 / 01期

关键词：

THEOPHYLLINE; IMMUNOASSAY; MONOCLONAL ANTIBODY;

D O I：

10.1016/0165-2478(91)90130-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Spleen cells from BALB/c mice immunised with KLH-theophylline conjugate were fused with a mouse myeloma cell line P3-X63-Ag8.653, and antibody-producing hybrids were identified by enzyme immunoassay. Three cell clones were obtained, each capable of producing a unique monoclonal antibody to theophylline. Using these monoclonal antibodies, an immunoassay system for theophylline was developed.

引用

页码：79 / 84

页数：6

共 6 条

[1] MEASUREMENT OF MONOCLONAL-ANTIBODY AFFINITY BY NONCOMPETITIVE ENZYME-IMMUNOASSAY [J].