NUCLEOTIDE-SEQUENCE AND STRUCTURE-ANALYSIS OF A CDNA-ENCODING AN ALPHA INSECT TOXIN FROM THE SCORPION LEIURUS-QUINQUESTRIATUS-HEBRAEUS

被引：31

作者：

GUREVITZ, M ^{[1
]}

URBACH, D ^{[1
]}

ZLOTKIN, E ^{[1
]}

ZILBERBERG, N ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM, INST LIFE SCI, IL-91904 JERUSALEM, ISRAEL

来源：

TOXICON | 1991年 / 29卷 / 10期

关键词：

D O I：

10.1016/0041-0101(91)90200-B

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A approximately 370 base pair cDNA encoding the alpha insect toxin Lqh-alpha-IT of the scorpion Leiurus quinquestriatus hebraeus was cloned and sequenced. The deduced amino acid sequence for the putative mature polypeptide is identical to the protein sequence determined chemically (EITAN et al., Biochemistry 29, 5941, 1990). A 19 amino acid signal peptide precedes the 64 amino acid long toxin. Two additional amino acid residues that do not correspond to the purified toxin are found at the COOH-terminus and may imply post-translational modification. The signal peptide region in the present clone differs obviously from that encoding the depressant insect toxin LqhIT2 derived from the same venom, but strongly resembles the leader peptide sequence of an alpha-mammal toxin from the scorpion Androctonus australis.

引用

页码：1270 / 1272

页数：3

共 13 条

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