ASSESSING ODOR GENERALIZATION IN THE RAT - A SENSITIVE TECHNIQUE

In investigating the ability of the rat to discriminate among urine odors from mice of different genetic strains, we developed a technique for determining the extent of odor generalization. Trained rats perform a discrete trial, go/no-go procedure, touching a bar in the presence of an S+ odor for water reward. Rats easily learned this task and restricted their responding to trials with the S+ stimulus, even though there was no penalty for responding to S-. However, when presented with test stimuli that might be more similar to S+ or S-, some rats responded only to the S+, and not at all to S- or any generalization stimuli. We then created series of stimuli composed of different levels of adulteration of the S+ stimulus with generalization stimuli. With these stimuli, rats produced graded levels of responding, allowing comparisons of similarity among odorant stimuli. This technique can be applied to other types of odorant stimuli, particularly biological compounds of unknown concentration or purity, and should be of use in an operant laboratory studying odorant perception, or odorant characteristics.