MODE OF ACTION OF ALANOSINE

Alanosine inhibits the synthesis of RNA adenine from formate-14C and from glycine-14C in Ehrlich ascites tumor cells in vivo. There is no appreciable depression of the rate of RNA guanine synthesis. The incorporation of preformed adenine-14C into RNA purines is unaffected. Inhibition of RNA adenine synthesis is not antagonized by glutamine or aspartate. The incorporation of thymidine-3H, uridine-3H, and l-leucine-14C into the acid-insoluble fraction of the cells in vitro is unaffected by the drug. Evidence indicates that alanosine may interfere with the conversion of inosine monophosphate to adenosine monophosphate. © 1968.