MAPPING AND CHARACTERIZATION OF 129 COSMIDS ON HUMAN-CHROMOSOME 11P

被引：7

作者：

CALL, KM

ITO, CY

LINDBERG, C

MEMISOGLU, A

PETROU, C

GLASER, T

JONES, C

HOUSMAN, DE

机构：

[1] UNIV N CAROLINA,CTR LINEBERGER CANC,CHAPEL HILL,NC 27514

[2] HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115

[3] E ROOSEVELT INST CANC RES,DENVER,CO

[4] MIT,CTR CANC RES,CAMBRIDGE,MA 02139

来源：

SOMATIC CELL AND MOLECULAR GENETICS | 1992年 / 18卷 / 05期

关键词：

D O I：

10.1007/BF01233086

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We constructed cosmid libraries from human-hamster somatic cell hybrids that possess all or part of the short arm of chromosome 11 as their only human complement and isolated 129 human 11p clones. These cosmids map to 22 of 25 intervals distinguished by a hybrid panel for chromosome 11p. Forty-eight single-copy sequences were subcloned from 25 cosmids. Six of 17 (35%) single-copy sequences tested identify 11 new polymorphisms. Restriction endonuclease analysis identified CpG islands in 16 of 68 cosmids (23.5%). Analysis of the distribution of restriction endonuclease sites recognizing CpG dinucleotides showed that clusters of these sites, including those associated with the 5' region of an 11p 13 Wilms' tumor gene, WT1, can span greater distances than generally recognized. The cosmids reported here should contribute to the construction of long-range physical maps and the isolation of additional genes on the short arm of chromosome 11.

引用

页码：463 / 475

页数：13

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