HPLC DETERMINATION OF D-GLUCARIC ACID IN HUMAN URINE

被引：11

作者：

POON, R

VILLENEUVE, DC

CHU, I

KINACH, R

机构：

[1] Bureau of Chemical Hazards, Health and Welfare Canada, Ottawa

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 1993年 / 17卷 / 03期

关键词：

D O I：

10.1093/jat/17.3.146

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An isocratic HPLC method has been developed for the direct measurement of d-glucaric acid in human urine. Pretreatment of urine with a boronic acid gel removed many interfering substances, including l-ascorbic acid and d-glucuronic acid. This method has a detection limit of 10µM d-glucaric acid (approximately 7 µmoles/g creatinine). The run-to-run precisions were 9.1% and 7.7% at urinary d-glucaric acid concentrations of 41 and 219 µmoles/g creatinine, respectively. Urinary d-glucaric acid concentrations in normal adults were found to cover a range of 15 to 89 µmoles/g creatinine (mean = 47 µmoles/g creatinine). The sensitivity of this method in detecting abnormal elevations in d-glucaric acid was demonstrated through its ability to measure changes in urinary concentrations with time after ingestion of d-glucuronolactone. © 1993 Oxford University Press.

引用

页码：146 / 150

页数：5

共 27 条

[1] EVIDENCE FOR FUNCTION OF D-GLUCARIC ACID AS AN INDICATOR FOR DRUG INDUCED ENHANCED METABOLISM THROUGH GLUCURONIC ACID PATHWAY IN MAN [J].