EVIDENCE OF A GROWTH HORMONE-RELEASING HORMONE-LIKE MOLECULE IN SALMON BRAIN, ONCORHYNCHUS-KETA AND ONCORHYNCUS-KISUTCH

被引：16

作者：

PARKER, DB

SHERWOOD, NM

机构：

[1] Department of Biology, University of Victoria, Victoria, BC V8W 2Y2

来源：

GENERAL AND COMPARATIVE ENDOCRINOLOGY | 1990年 / 79卷 / 01期

关键词：

D O I：

10.1016/0016-6480(90)90092-Z

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

An antiserum with conformational specificity to human growth hormone-releasing hormone (hGH-RH 1-44 NH2) was produced and used to develop a radioimmunoassay to detect immunoreactive (ir) GH-RH in brain extracts of salmon, guinea pig, and mouse. Evidence of an immunoreactive GH-RH from salmon brain extracts with a retention time on reverse-phase high-performance liquid chromatography (HPLC) distinct from hGH-RH 1-44 is presented. Salmon irGH-RH from crude extracts elutes 1-2 min earlier than hGH-RH 1-44 (NH2) in a gradient HPLC system, whereas affinity-purified salmon irGH-RH elutes 12-13 min earlier in a near-isocratic system, suggesting that the salmon molecule is more hydrophilic. © 1990.

引用

页码：95 / 102

页数：8

共 13 条

[1] PARTIAL-PURIFICATION AND CHARACTERIZATION OF A NOVEL GROWTH HORMONE-RELEASING FACTOR (GRF) FROM TELEOST BRAIN RELATED TO THE RAT HYPOTHALAMIC PEPTIDE [J].