GENETICALLY-ENGINEERED SUBUNIT VACCINE AGAINST FELINE LEUKEMIA-VIRUS - PROTECTIVE IMMUNE-RESPONSE IN CATS

被引：74

作者：

MARCIANI, DJ ^{[1
]}

KENSIL, CR ^{[1
]}

BELTZ, GA ^{[1
]}

HUNG, CH ^{[1
]}

CRONIER, J ^{[1
]}

AUBERT, A ^{[1
]}

机构：

[1] VIRBAC LABS,F-06516 CARROS,FRANCE

来源：

VACCINE | 1991年 / 9卷 / 02期

关键词：

RECOMBINANT; FELINE LEUKEMIA VIRUS; ADJUVANTS; SAPONIN; ENVELOPE GLYCOPROTEINS;

D O I：

10.1016/0264-410X(91)90262-5

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A recombinant retroviral subunit vaccine has been developed that successfully protects cats from infectious feline leukaemia virus (FeLV) challenge. The antigen used is a non-glycosylated protein derived from the envelope glycoprotein of FeLV subgroup A, expressed in Escherichia coli. This recombinant protein, rgp70D, includes the entire exterior envelope protein gp70, plus the first 34 amino acids from the transmembrane protein p15E. The vaccine consists of purified rgp70D absorbed on to aluminium hydroxide and used in conjunction with a novel saponin adjuvant. Cats immunized with this formulation developed a strong humoral immune response, including neutralizing and feline oncornavirus-associated cell membrane antigen antibodies. Vaccinated animals showed an anamnestic response upon intraperitoneal challenge with FeLV-A, and were protected from viral infection. In contrast, the control animals developed viraemia shortly after the challenge, which in most cases became chronic. Formulation of the same antigen with other widely used adjuvants elicited poor protective immune responses in cats.

引用

页码：89 / 96

页数：8

共 36 条

[1] AN ADJUVANT FORMULATION THAT SELECTIVELY ELICITS THE FORMATION OF ANTIBODIES OF PROTECTIVE ISOTYPES AND OF CELL-MEDIATED-IMMUNITY [J].