IMMUNO(CYTO)CHEMICAL CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO GILTHEAD SEABREAM (SPARUS-AURATA) IMMUNOGLOBULIN

被引：30

作者：

NAVARRO, V

QUESADA, JA

ABAD, ME

TAVERNE, N

ROMBOUT, JHWM

机构：

[1] Department of Cell Biology, Faculty of Biology, University of Murcia

[2] Department of Experimental Animal Morphology and Cell Biology, Agricultural University, 6700 AH, Wageningen

来源：

FISH & SHELLFISH IMMUNOLOGY | 1993年 / 3卷 / 03期

关键词：

MONOCLONAL ANTIBODIES; IGM; SPARUS-AURATA; LYMPHOCYTE SUBPOPULATIONS; SPLEEN; THYMUS; HEAD KIDNEY;

D O I：

10.1006/fsim.1993.1017

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Monoclonal antibodies (MAbs) were produced against gilthead seabream Ig, and their reactivity was demonstrated by immunofluorescence microscopy, flow cytometry, immuno-electronmicroscopy and immunochemistry. Only one of these MAbs (WSI 5) appeared to react with living B cells and was selected for further research. WSI 5 was reactive with the L chain (± 25 kDa) of both forms of Ig found in gilthead seabream serum: a tetrameric (± 760 kDa) and a monomeric (± 190 kDa) molecule. Immunofluorescence reactions on cytocentrifuge slides and cryostat tissue sections showed Ig-bearing lymphocytes and Ig-containing plasma cells in head kidney, spleen and thymus. FACS-analysis of head kidney, spleen and thymus revealed about 22%, 23% and 3% of Ig-surface positive B cells, respectively. Immunogold labelling of living cells showed that Ig was frequently present in small clusters at the external membrane of Ig-bearing and Ig-containing cells. © 1993 by Academic Press, Inc.

引用

页码：167 / 177

页数：11

共 17 条

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