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CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF CYCLODEXTRIN GLUCANOTRANSFERASE FROM ALKALOPHILIC BACILLUS SP-1011

被引：13
作者：
HAGA, K
HARATA, K
NAKAMURA, A
YAMANE, K
机构：
[1] UNIV TSUKUBA,INST BIOL SCI,TSUKUBA,IBARAKI 305,JAPAN
[2] NATL INST BIOSCI & HUMAN TECHNOL,TSUKUBA,IBARAKI,JAPAN
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 237卷 / 01期
关键词：
ALKALOPHILIC BACILLUS; CRYSTALLIZATION CYCLODEXTRIN GLUCANOTRANSFERASE; X-RAY CRYSTALLOGRAPHY;
D O I：
10.1006/jmbi.1994.1216
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Large crystals of cyclodextrin glucanotransferase (CGTase) from alkalophilic Bacillus sp. 1011 , a typical alkalophilic enzyme, have been obtained at room temperature using polyethylene glycol 3000 and 2-propanol as precipitant. They belong to the triclinic space group P1 with the following unit cell constants: a = 64.93 Å, b = 74.45 Å, c = 79.12 Å, α = 85.2° , β = 105.0° and γ = 101.0°. The crystallographic asymmetric unit seems to contain two molecules of CGTase, with crystal volume per protein mass (V(m)) of 2.41 Å3/Da and solvent content of 49% by volume. The crystals diffract to at least 2.0 Å resolution and they are suitable for X-ray analysis.
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页码：163 / 164
页数：2
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