CHEMILUMINESCENT UNIVERSAL PROBE FOR BACTERIAL RIBOTYPING

被引：8

作者：

GUSTAFERRO, CA

PERSING, DH

机构：

[1] MAYO CLIN & MAYO FDN,DEPT LAB MED & PATHOL,CLIN MICROBIOL SECT,ROCHESTER,MN 55905

[2] MAYO CLIN & MAYO FDN,DEPT INTERNAL MED,INFECT DIS SECT,ROCHESTER,MN 55905

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 04期

关键词：

D O I：

10.1128/JCM.30.4.1039-1041.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe a novel procedure for direct covalent coupling of horseradish peroxidase to rRNA and ribotyping by using enhanced chemiluminescence. Compared with their P-32-end-labeled counterparts, chemiluminescent rRNA probes are stable and easy to synthesize and provide results as good as or superior to those obtained with isotopic labeling. Direct chemiluminescent labeling of Escherichia coli rRNA produces a sensitive, universal probe suitable for clinical laboratory use in the investigation of nosocomial outbreaks.

引用

页码：1039 / 1041

页数：3

共 17 条

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