QUANTITATION OF BENZOYLNORECGONINE AND OTHER COCAINE METABOLITES IN MECONIUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：31

作者：

MURPHEY, LJ

OLSEN, GD

KONKOL, RJ

机构：

[1] OREGON HLTH SCI UNIV,SCH MED,DEPT PHARMACOL,L221,3181 SW SAM JACKSON PK RD,PORTLAND,OR 97201

[2] OREGON HLTH SCI UNIV,SCH MED,DEPT NEUROL,PORTLAND,OR 97201

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 613卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80150-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for simultaneous extraction of cocaine and metabolites benzoylnorecgonine, benzoylecgonine and norcocaine from meconium was developed. The procedure uses solid-phase extraction columns with both cation-exchange and hydrophobic properties after vortex-mixing meconium with methanol. Chromatography utilizes reversed-phase high-performance liquid chromatography with a C18 column and phosphate buffer-acetonitrile as mobile phase. The method is specific and sensitive to 50 ng/g meconium for all compounds. Standard curves are linear from 0.05 to 5.0 mug/g (r2 greater-than-or-equal-to 0.989). Intra-assay coefficients of variation were less-than-or-equal-to 6.9%. Meconium from infants exposed to cocaine in utero contained varying combinations of the four drugs.

引用

页码：330 / 335

页数：6

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