EFFECTS OF SAMPLE PROBE SURFACE-TEMPERATURE ON THE DETECTION OF PEPTIDES BY FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY

被引：3

作者：

PAPAYANNOPOULOS, IA ^{[1
]}

BIEMANN, K ^{[1
]}

机构：

[1] MIT,DEPT CHEM,ROOM 56-010,CAMBRIDGE,MA 02139

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES | 1992年 / 122卷

关键词：

PEPTIDES; PROBE SURFACE TEMPERATURE; SUPPRESSION EFFECTS;

D O I：

10.1016/0168-1176(92)87006-Z

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

It has been observed that in the analysis of peptide mixtures by fast atom bombardment mass spectrometry some of the components are not detected and, usually, the ion yields of protonated peptide molecules do not reflect their relative molar abundance. This phenomenon has been attributed to the suppression of ionization of some of the mixture components by other components. It has been found that a change in the sample probe surface temperature, effected with a thermoelectrically-cooled probe, eliminates the suppression effects and, in some instances, even reverses them.

引用

页码：43 / 50

页数：8

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