CIRCULATING GROWTH-HORMONE ISOFORMS IN GIRLS WITH TURNERS-SYNDROME

被引:16
作者
BLETHEN, SL
ALBERTSSONWIKLAND, K
FAKLIS, EJ
CHASALOW, FI
机构
[1] UNIV GOTHENBURG, DEPT PEDIAT, INT PEDIAT GROWTH RES CTR, GOTHENBURG, SWEDEN
[2] LONG ISL JEWISH MED CTR, SCHNEIDER CHILDRENS HOSP, DEPT PEDIAT, NEW HYDE PK, NY 11042 USA
[3] MAIMONIDES HOSP, DEPT PEDIAT, BROOKLYN, NY 11219 USA
关键词
D O I
10.1210/jc.78.6.1439
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To evaluate the presence of different GH isoforms in serum of girls with Turner's syndrome, we measured the serum GH content using RIAs with three different site-specific monoclonal antibodies (MAbs). We compared the results to those obtained with authentic GH and GH isoforms. Compared to pituitary GH (mol wt, 22K daltons) as the standard for all three MAbs, serum from girls with Turner's syndrome did not displace tracer [I-125]GH equally with all-three MAbs. The relative amounts of GH-immunoreactive material found in Turner's syndrome were different from the amounts observed in normal adults and most children with idiopathic short stature. The presence of GH, other than 22K GH, in serum from girls with Turner's syndrome was confirmed by affinity chromatography. The existence of different isoforms of GH, as shown by different cross-reactivity patterns with different MAbs to GH, may explain the conflicting results reported for GH secretion in girls with Turner's syndrome.
引用
收藏
页码:1439 / 1443
页数:5
相关论文
共 25 条
[1]  
ALBERTSSONWIKLA.K, 1991, EXCERPTA MED ICS, V924, P23
[2]  
ALBERTSSONWIKLAND K, 1990, TURNER SYNDROME, P233
[3]  
BAUMAN G, 1986, J CLIN ENDOCR METAB, V62, P143
[4]   GROWTH-HORMONE HETEROGENEITY - GENES, ISOHORMONES, VARIANTS, AND BINDING-PROTEINS [J].
BAUMANN, G .
ENDOCRINE REVIEWS, 1991, 12 (04) :424-449
[5]  
BLETHEN S L, 1989, Journal of Pediatric Endocrinology, V3, P217
[6]   USE OF A 2-SITE IMMUNORADIOMETRIC ASSAY FOR GROWTH-HORMONE (GD) IN IDENTIFYING CHILDREN WITH GH-DEPENDENT GROWTH FAILURE [J].
BLETHEN, SL ;
CHASALOW, FI .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1983, 57 (05) :1031-1035
[7]  
BLETHEN SL, 1988, 70TH P ANN M END SOC
[8]   VARIABILITY IN THE QUANTITATION OF CIRCULATING GROWTH-HORMONE USING COMMERCIAL IMMUNOASSAYS [J].
CELNIKER, AC ;
CHEN, AB ;
WERT, RM ;
SHERMAN, BM .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1989, 68 (02) :469-476
[9]   STRUCTURAL VARIANTS OF HUMAN GROWTH-HORMONE - BIOCHEMICAL, GENETIC, AND CLINICAL ASPECTS [J].
CHAWLA, RK ;
PARKS, JS ;
RUDMAN, D .
ANNUAL REVIEW OF MEDICINE, 1983, 34 :519-547
[10]   RECEPTOR AND ANTIBODY EPITOPES IN HUMAN GROWTH-HORMONE IDENTIFIED BY HOMOLOG-SCANNING MUTAGENESIS [J].
CUNNINGHAM, BC ;
JHURANI, P ;
NG, P ;
WELLS, JA .
SCIENCE, 1989, 243 (4896) :1330-1336