IDENTIFICATION OF A P-GLYCOPROTEIN-RELATED PROTEIN (MINI-P-GLYCOPROTEIN) WHICH IS OVEREXPRESSED IN MULTIDRUG-RESISTANT CELLS

被引：20

作者：

KAWAI, K ^{[1
]}

KUSANO, I ^{[1
]}

IDO, M ^{[1
]}

SAKURAI, M ^{[1
]}

SHIRAISHI, T ^{[1
]}

YATANI, R ^{[1
]}

机构：

[1] MIE UNIV,SCH MED,DEPT PEDIAT,TSU,MIE 514,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 198卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.1115

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Drug resistant variant cell lines were selected in vitro for resistance to adriamycin or vincristine, P388/ADR and P388/VCR-600, respectively, from P388 murine leukemia cells. These cells were demonstrated by immunoblot studies with P-glycoprotein specific monoclonal antibody (C219) to overexpress not only P-glycoprotein but also approximately 65 kDa protein, and the expression levels were found to correlate with the degree of resistance. Furthermore, in Northern blot analysis with an MDR1 cDNA as a probe, an overexpressed transcript with the length of about 2.4 kilobases which might encode this previously uncharacterized protein was also detected in the multidrug resistant cell line. Thus, it is suggested that this protein might be associated with multidrug resistant phenotype and be related to P-glycoprotein. © 1994 Academic Press, Inc.

引用

页码：804 / 810

页数：7

共 22 条

[1]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[2] THE BIOCHEMISTRY OF P-GLYCOPROTEIN-MEDIATED MULTIDRUG RESISTANCE [J].