SENSITIVE NONISOTOPIC DNA HYBRIDIZATION ASSAY OR IMMEDIATE-EARLY ANTIGEN-DETECTION FOR RAPID IDENTIFICATION OF HUMAN CYTOMEGALOVIRUS IN URINE

被引：12

作者：

KIMPTON, CP ^{[1
]}

MORRIS, DJ ^{[1
]}

CORBITT, G ^{[1
]}

机构：

[1] UNIV MANCHESTER,SCH MED,DEPT PATHOL SCI,DIV VIROL,STOPFORD BLDG,OXFORD RD,MANCHESTER M13 9PT,LANCS,ENGLAND

来源：

JOURNAL OF VIROLOGICAL METHODS | 1991年 / 32卷 / 01期

关键词：

CYTOMEGALOVIRUS; NONISOTOPIC DNA HYBRIDIZATION; IMMEDIATE-EARLY ANTIGEN DETECTION; COMPARISON OF METHODS;

D O I：

10.1016/0166-0934(91)90188-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive non-radioactive DNA hybridisation assay employing digoxigenin-labelled probes was compared with immediate-early antigen detection and conventional virus isolation for the identification of human cytomegalovirus (HCMV) in 249 urine samples. Of 44 specimens yielding HCMV by virus isolation, more were positive by DNA hybridisation (32; 73%) than by immediate-early antigen detection (25; 52%) (P = 0.05). The specificity of the hybridisation assay in 45 apparently falsely positive specimens was supported by detection of HCMV DNA in 40 of these specimens using the polymerase chain reaction. Many urine specimens may thus contain large amounts of non-viable virus or free viral DNA. Evaluation of various protocols for the extraction and denaturation of virus DNA prior to hybridisation showed that proteinase K digestion with phenol/chloroform extraction was the most sensitive and reliable procedure. We conclude that the non-radioactive DNA hybridisation assay described is a potentially valuable routine diagnostic test.

引用

页码：89 / 99

页数：11

共 25 条

[1] EARLY DIAGNOSIS OF CYTOMEGALO-VIRUS INFECTION IN RENAL-TRANSPLANT AND DIALYSIS PATIENTS BY DNA-DNA HYBRIDIZATION ASSAY [J].