INVITRO LIGAND-BINDING OF I-125 RECOMBINANT HUMAN ACTIVIN-A TO THE FEMALE RAT-BRAIN

被引：20

作者：

JAKEMAN, L ^{[1
]}

MATHER, J ^{[1
]}

WOODRUFF, T ^{[1
]}

机构：

[1] GENENTECH INC, DEPT CELL CULTURE, R&D, S SAN FRANCISCO, CA 94080 USA

来源：

ENDOCRINOLOGY | 1992年 / 131卷 / 06期

关键词：

D O I：

10.1210/en.131.6.3117

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Activin has been localized within cells and terminals in the brain. However, little is known about the site of action of this hormone within the brain. In the present study in vitro ligand autoradiography was used to determine the distribution of high affinity binding sites for I-125-rh activin A in rat brain. The highest density of binding sites were concentrated in the amygdala and other forebrain limbic structures. Binding was saturable in selected amygdala nuclei with an apparent Kd of approximately 0.2 nM. I-125-rh activin A binds specifically in regions known to contain terminals immunoreactive for this hormone, and also at sites distinct from the location of activin A containing neuronal terminals (e.g. the ventral limbic system).

引用

页码：3117 / 3119

页数：3

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