FUNCTIONAL EXPRESSION OF GASTRIC H,K-ATPASE USING THE BACULOVIRUS EXPRESSION SYSTEM

被引：61

作者：

KLAASSEN, CHW ^{[1
]}

VANUEM, TJF ^{[1
]}

DEMOEL, MP ^{[1
]}

DECALUWE, GLJ ^{[1
]}

SWARTS, HGP ^{[1
]}

DEPONT, JJHHM ^{[1
]}

机构：

[1] CATHOLIC UNIV NIJMEGEN,DEPT BIOCHEM,POB 9101,6500 HB NIJMEGEN,NETHERLANDS

来源：

FEBS LETTERS | 1993年 / 329卷 / 03期

关键词：

H; K-ATPASE; BACULOVIRUS EXPRESSION SYSTEM; SF9; CELL; IN-VITRO EXPRESSION; SCH-28080;

D O I：

10.1016/0014-5793(93)80237-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel approach to construct a single recombinant baculovirus expressing two protein subunits simultaneously by replacing polyhedrin as well as p10 coding sequences is described. The recombinant baculovirus expressed the alpha- as well as the beta-subunit of the gastric H,K-ATPase. Sf9 cells infected with this virus exhibited a K+- and SCH 28080-sensitive ATP-dependent phosphorylation capacity in purified Sf9 membranes similar to native H,K-ATPase. This activity was not present in control membranes containing only one of the two H,K-ATPase subunits. We therefore conclude that both subunits are essential for the phosphorylation capacity of H,K-ATPase.

引用

页码：277 / 282

页数：6

共 34 条

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