SENSITIVE AND SPECIFIC POLYMERASE CHAIN-REACTION ASSAYS FOR DETECTION OF BORDETELLA-PERTUSSIS IN NASOPHARYNGEAL SPECIMENS

被引:45
作者
HE, QS [1 ]
MERTSOLA, J [1 ]
SOINI, H [1 ]
VILJANEN, MK [1 ]
机构
[1] UNIV TURKU, TURKU UNIV HOSP, DEPT PEDIAT, TURKU, FINLAND
基金
芬兰科学院;
关键词
D O I
10.1016/S0022-3476(94)70365-5
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Polymerase chain reaction (PCR) assays that amplify segments of a repeated gene element and a toxin promoter gene of Bordetella pertussis were compared with a culture established for the diagnosis of pertussis. Of 44 nasopharyngeal (NP) aspirates collected during a pertussis outbreak, repeated gene element PCR showed a positive result in 21 (48%), including all three patients with positive culture results. Results of toxin promoter gene PCR were positive in eight (18%) cases, and the pathogen was not detected in one patient with a positive culture result. A more sensitive nested PCR assay, based on repeated gene element PCR, was then developed. During a second outbreak two different transportation systems were tested in 146 duplicate NP swabs. Transportation of swabs in empty tubes proved to be better than in transport media for PCR. A total of 190 NP specimens from the two outbreaks were tested, and in 56 the results were shown to be positive by PCR, including all 16 cases confirmed as positive by culture. We conclude that the PCR assay is more sensitive than culture in the diagnosis of pertussis; NP swabbing is a simple, practical, and reliable method of collecting clinical specimens for PCR assays and cultures.
引用
收藏
页码:421 / 426
页数:6
相关论文
共 22 条
  • [1] RAPID DIAGNOSIS OF HERPES-SIMPLEX ENCEPHALITIS BY NESTED POLYMERASE CHAIN-REACTION ASSAY OF CEREBROSPINAL-FLUID
    AURELIUS, E
    JOHANSSON, B
    SKOLDENBERG, B
    STALAND, A
    FORSGREN, M
    [J]. LANCET, 1991, 337 (8735) : 189 - 192
  • [2] IDENTIFICATION OF BORDETELLA-PERTUSSIS IN NASOPHARYNGEAL SWABS BY PCR AMPLIFICATION OF A REGION OF THE ADENYLATE-CYCLASE GENE
    DOUGLAS, E
    COOTE, JG
    PARTON, R
    MCPHEAT, W
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 1993, 38 (02) : 140 - 144
  • [3] EISENSTEIN BI, 1990, NEW ENGL J MED, V322, P178
  • [4] FINE PEM, 1982, LANCET, V1, P666
  • [5] ANALYSIS OF A REPETITIVE DNA-SEQUENCE FROM BORDETELLA-PERTUSSIS AND ITS APPLICATION TO THE DIAGNOSIS OF PERTUSSIS USING THE POLYMERASE CHAIN-REACTION
    GLARE, EM
    PATON, JC
    PREMIER, RR
    LAWRENCE, AJ
    NISBET, IT
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (09) : 1982 - 1987
  • [6] COMPARISON OF POLYMERASE CHAIN-REACTION WITH CULTURE AND ENZYME-IMMUNOASSAY FOR DIAGNOSIS OF PERTUSSIS
    HE, QS
    MERTSOLA, J
    SOINI, H
    SKURNIK, M
    RUUSKANEN, O
    VILJANEN, MK
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (03) : 642 - 645
  • [7] SPECIFIC IDENTIFICATION OF BORDETELLA-PERTUSSIS BY THE POLYMERASE CHAIN-REACTION
    HOUARD, S
    HACKEL, C
    HERZOG, A
    BOLLEN, A
    [J]. RESEARCH IN MICROBIOLOGY, 1989, 140 (07) : 477 - 487
  • [8] KOWK S, 1989, NATURE, V339, P237
  • [9] KWANTES W, 1983, J HYG, V99, P149
  • [10] IMPROVED DIAGNOSIS OF PNEUMOCYSTIS-CARINII INFECTION BY POLYMERASE CHAIN-REACTION ON INDUCED SPUTUM AND BLOOD
    LIPSCHIK, GY
    GILL, VJ
    LUNDGREN, JD
    ANDRAWIS, VA
    NELSON, NA
    NIELSEN, JO
    OGNIBENE, FP
    KOVACS, JA
    [J]. LANCET, 1992, 340 (8813) : 203 - 206