DETERMINATION OF PROSTAGLANDINS AND THROMBOXANE IN WHOLE-BLOOD BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORIMETRIC DETECTION

被引:24
作者
ENGELS, W [1 ]
KAMPS, MAF [1 ]
LEMMENS, PJMR [1 ]
VANDERVUSSE, GJ [1 ]
RENEMAN, RS [1 ]
机构
[1] STATE UNIV LIMBURG, DEPT PHYSIOL, 6200 MD MAASTRICHT, NETHERLANDS
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1988年 / 427卷 / 02期
关键词
D O I
10.1016/0378-4347(88)80123-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A highly sensitive and specific assay for the quantitation of prostaglandins (PGs) such as PGE1, PGE2, PGF1.alpha., 6-keto-PGF1.alpha., and including thromoxane B2, is described. The method involves the addition of PGF1.alpha. and PGE as the internal standards, extraction from whole blood and purification by silica gel column chromatography. Following conversion into the methoximes, purfication by reversed-phase chromatography and esterfication withpanacyl bromide, samples are analysed by high-performance liquid chromatography with fluorimetric detection. The lower limit of detection of the eicosanoids 6-keto-PGF1.alpha., thromboxane B2 and PGF2.alpha. in blood is ca. 50 pg/ml and that of PGE2 is 100 pg/ml. Assay linearity is demonstrated over a range from 60 pg to 60 ng of eicosanodi injected. The method allows simultaneous assessment of prostaglandins and thromboxane extracted from complex biological fluids at picogram levels.
引用
收藏
页码:209 / 218
页数:10
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