INCREASED PROENKEPHALIN MESSENGER-RNA LEVELS IN THE RAT NEOSTRIATUM FOLLOWING LESION OF THE IPSILATERAL NIGROSTRIATAL DOPAMINE PATHWAY WITH 1-METHYL-4-PHENYLPYRIDINIUM ION (MPP+) - REVERSAL BY EMBRYONIC NIGRAL DOPAMINE GRAFTS

被引:34
作者
SIRINATHSINGHJI, DJS [1 ]
DUNNETT, SB [1 ]
机构
[1] UNIV CAMBRIDGE, DEPT EXPTL PSYCHOL, CAMBRIDGE, ENGLAND
来源
MOLECULAR BRAIN RESEARCH | 1991年 / 9卷 / 03期
关键词
PROENKEPHALIN MESSENGER RNA; NEOSTRIATUM; INSITU HYBRIDIZATION; 1-METHYL-4-PHENYLPYRIDINIUM ION; NIGROSTRIATAL DOPAMINE PATHWAY; NEURAL TRANSPLANTATION; EMBRYONIC SUBSTANTIA NIGRA; RAT;
D O I
10.1016/0169-328X(91)90011-L
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In situ hybridization studies were performed to study the changes in proenkephalin mRNA levels in the neostriatum of rats with long-term (18 months) unilateral lesions of the nigrostriatal dopamine (DA) pathway induced by 1-methyl-4-phenylpyridinium ion (MPP+) and in animals bearing embryonic DA grafts implanted into the DA depleted striatum. In the ipsilateral striatum of MPP+ -lesioned animals, there was a 2-fold increase in the levels of proenkephalin mRNA compared with those in the contralateral striatum of the same animals or the ipsilateral striatum of control animals. High resolution analysis using emulsion autoradiography showed that increase in proenkephalin gene expression in response to DA-denervation by MPP+ was due to an increase in the hybridization signal over individual expressing cells as well as to an increase in the number of labelled cells. In the DA-grafted striatum the levels of proenkephalin mRNA were significantly (P < 0.01) reduced when compared with those in the MPP+ -lesioned striatum due to both a decrease in the number of labelled cells as well as the hybridization density per individual cell. Moreover, when compared with the ipsilateral striatum of control animals, the levels of proenkephalin mRNA in the DA-grafted striatum was slightly lower due to a 20% decrease in the number of labelled cells rather than a decrease in the hybridization signal per individual cell. The results of this study are important in two respects. Firstly, they clearly show that the increase in proenkephalin gene expression in the striatum of rats with complete nigrostriatal DA lesions, can be maintained for many months after the lesion. Secondly, this increase in proenkephalin gene expression can be completely reversed by embryonic DA grafts which extensively reinnervate the DA-depleted striatum and make synaptic connections with the intrinsic striatal enkephalinergic neurons. The results indicate that the nigral DA neurons exert a tonic inhibitory influence on the activity of proenkephalin mRNA-containing neurons in the straitum.
引用
收藏
页码:263 / 269
页数:7
相关论文
共 39 条
  • [1] REDUCTION OF STRIATAL DOPAMINERGIC NEUROTRANSMISSION ELEVATES STRIATAL PROENKEPHALIN MESSENGER-RNA
    ANGULO, JA
    DAVIS, LG
    BURKHART, BA
    CHRISTOPH, GR
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 1986, 130 (03) : 341 - 343
  • [2] CELLULAR-LOCALIZATION OF ENKEPHALIN GENE-EXPRESSION IN MPTP-TREATED CYNOMOLGUS MONKEYS
    AUGOOD, SJ
    EMSON, PC
    MITCHELL, IJ
    BOYCE, S
    CLARKE, CE
    CROSSMAN, AR
    [J]. MOLECULAR BRAIN RESEARCH, 1989, 6 (01): : 85 - 92
  • [3] EXPERIMENTAL HEMIPARKINSONISM IN THE RAT FOLLOWING CHRONIC UNILATERAL INFUSION OF MPP+ INTO THE NIGROSTRIATAL DOPAMINE PATHWAY .2. DIFFERENTIAL LOCALIZATION OF DOPAMINE AND CHOLECYSTOKININ RECEPTORS
    BERESFORD, IJM
    DAVENPORT, AP
    SIRINATHSINGHJI, DJS
    HALL, MD
    HILL, RG
    HUGHES, J
    [J]. NEUROSCIENCE, 1988, 27 (01) : 129 - 143
  • [4] MECHANISMS OF ACTION OF INTRACEREBRAL NEURAL IMPLANTS - STUDIES ON NIGRAL AND STRIATAL GRAFTS TO THE LESIONED STRIATUM
    BJORKLUND, A
    LINDVALL, O
    ISACSON, O
    BRUNDIN, P
    WICTORIN, K
    STRECKER, RE
    CLARKE, DJ
    DUNNETT, SB
    [J]. TRENDS IN NEUROSCIENCES, 1987, 10 (12) : 509 - 516
  • [5] BJORKLUND A, 1983, ACTA PHYSIOL SCAND, P1
  • [6] Bjorklund A., 1986, HDB PHYSL NERVOUS SY, V4, P155
  • [7] A TYPE OF ASPINY NEURON IN THE RAT NEOSTRIATUM ACCUMULATES [H-3]GAMMA-AMINOBUTYRIC ACID - COMBINATION OF GOLGI-STAINING, AUTORADIOGRAPHY, AND ELECTRON-MICROSCOPY
    BOLAM, JP
    CLARKE, DJ
    SMITH, AD
    SOMOGYI, P
    [J]. JOURNAL OF COMPARATIVE NEUROLOGY, 1983, 213 (02) : 121 - 134
  • [8] GLUTAMATE DECARBOXYLASE-IMMUNOREACTIVE STRUCTURES IN THE RAT NEOSTRIATUM - A CORRELATED LIGHT AND ELECTRON-MICROSCOPIC STUDY INCLUDING A COMBINATION OF GOLGI IMPREGNATION WITH IMMUNOCYTOCHEMISTRY
    BOLAM, JP
    POWELL, JF
    WU, JY
    SMITH, AD
    [J]. JOURNAL OF COMPARATIVE NEUROLOGY, 1985, 237 (01) : 1 - 20
  • [9] BOLAM JP, 1987, EXP BRAIN RES, V68, P131
  • [10] HUMAN-FETAL DOPAMINE NEURONS GRAFTED IN A RAT MODEL OF PARKINSONS-DISEASE - ULTRASTRUCTURAL EVIDENCE FOR SYNAPSE FORMATION USING TYROSINE-HYDROXYLASE IMMUNOCYTOCHEMISTRY
    CLARKE, DJ
    BRUNDIN, P
    STRECKER, RE
    NILSSON, OG
    BJORKLUND, A
    LINDVALL, O
    [J]. EXPERIMENTAL BRAIN RESEARCH, 1988, 73 (01) : 115 - 126