CYTOGENETIC STUDY OF BOVINE OOCYTES MATURED IN-VITRO

被引：21

作者：

ECTORS, FJ ^{[1
]}

KOULISCHER, L ^{[1
]}

JAMAR, M ^{[1
]}

HERENS, C ^{[1
]}

VERLOES, A ^{[1
]}

REMY, B ^{[1
]}

BECKERS, JF ^{[1
]}

机构：

[1] STATE UNIV LIEGE, FAC MED, CTR HUMAN GENET, SART, BELGIUM

来源：

THERIOGENOLOGY | 1995年 / 44卷 / 03期

关键词：

IN VITRO MATURATION; MEIOTIC CHROMOSOMES; BOVINE OOCYTES; IN VITRO FERTILIZATION;

D O I：

10.1016/0093-691X(95)00198-H

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Described in the present paper is a cytogenetic study of bovine oocytes matured in vitro. The cumulus-oocyte complexes (COG), punctured from ovaries recovered in a local slaughterhouse, were classified into 3 groups according to follicular diameter: 1 to 4mm, 5 to 8mm and 9 to 13 mm. Metaphases available for observation were classified as metaphase I, haploid and diploid metaphase II. High levels of haploid metaphases II (90.6, 86.9 and 94.4 %) among the 3 groups of follicular sizes indicated successful meiotic resumption during in vitro maturation and suggested that cytoplasmic maturation may be responsible for low developmental rate after IVM, IVF and in vitro development (IVD).

引用

页码：445 / 450

页数：6

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