USE OF REPETITIVE DNA PROBES AS PHYSICAL MAPPING STRATEGY IN CAENORHABDITIS-ELEGANS

被引：9

作者：

CANGIANO, G ^{[1
]}

AMEER, H ^{[1
]}

WATERSTON, R ^{[1
]}

LAVOLPE, A ^{[1
]}

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT GENET,ST LOUIS,MO 63110

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 17期

关键词：

D O I：

10.1093/nar/18.17.5077

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for linking genomic sequences cloned in yeast artificial chromosomes (YACs) has been tested using Caenorhabditis elegans as a model system. Yeast clones carrying YACs with repeated sequences were selected from a C. elegans genomic library, total DNA was digested with restriction enzymes, transferred to nylon membranes and probed with a variety of repetitive DNA probes. YAC clones that overlap share common bands with one or more repetitive DNA probes. In 159 YAC clones tested with one restriction enzyme and six probes 28 overlapping clones were detected. The advantages and limitations of this method for construction of YAC physical maps is discussed. © 1990 Oxford University Press.

引用

页码：5077 / 5081

页数：5

共 12 条

[1] CONSTRUCTION OF YEAST ARTIFICIAL CHROMOSOME LIBRARIES WITH LARGE INSERTS USING FRACTIONATION BY PULSED-FIELD GEL-ELECTROPHORESIS [J].