PROTEIN-KINASE-C IS PRESENT IN HUMAN SPERM - POSSIBLE ROLE IN FLAGELLAR MOTILITY

被引：92

作者：

ROTEM, R

PAZ, GF

HOMONNAI, ZT

KALINA, M

NAOR, Z

机构：

[1] TEL AVIV MED CTR & SCH MED,SERLIN MATERN HOSP,INST STUDY FERTIL,IL-61070 TEL AVIV,ISRAEL

[2] TEL AVIV UNIV,SACKLER FAC MED,DEPT HISTOL & CELL BIOL,IL-69978 TEL AVIV,ISRAEL

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 18期

关键词：

Immunohistochemistry; Phorbol ester;

D O I：

10.1073/pnas.87.18.7305

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the presence of protein kinase C (PKC) in ejaculated human sperm as revealed by enzymatic activity assay and indirect immunohistochemistry. PKC is localized in the equatorial segment and in the principal piece of the tail. Addition of phorbol 12-myristate 13-acetate resulted in increased flagellar motility that was blocked by known PKC inhibitors such as sphingosine, staurosporine, and 1-(5-isoquinoylinylsulfonyl)-2-methylpiperazine. A very good correlation (r = 0.9, P < 0.001) was found between the percentage of PKC-stained sperm cells and motility. We propose that PKC is involved in the regulation of flagellar motility in human sperm.

引用

页码：7305 / 7308

页数：4

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