DEGRADATION OF 4-NITROBENZOATE VIA 4-HYDROXYLAMINOBENZOATE AND 3,4-DIHYDROXYBENZOATE IN COMAMONAS-ACIDOVORANS NBA-10

被引：48

作者：

GROENEWEGEN, PEJ ^{[1
]}

DEBONT, JAM ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN,DEPT FOOD SCI,DIV IND MICROBIOL,POB 8129,6700 EV WAGENINGEN,NETHERLANDS

来源：

ARCHIVES OF MICROBIOLOGY | 1992年 / 158卷 / 05期

关键词：

4-NITROBENZOATE; 4-HYDROXYLAMINOBENZOATE; 3,4-DIHYDROXYBENZOATE; NITROAROMATIC COMPOUNDS; COMAMONAS-ACIDOVORANS;

D O I：

10.1007/BF00245369

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Comamonas acidovorans NBA-10 was previously shown to degrade 4-nitrobenzoate via 4-hydroxylaminobenzoate and 3,4-dihydroxybenzoate. Washed cells, grown on a mixture of 4-nitrobenzoate and ethanol, stoichiometrically produced ammonium and 3,4-dihydroxybenzoate from 4-nitrobenzoate under anaerobic conditions provided ethanol was present. In cell extracts 4-hydroxylaminobenzoate was degraded to ammonium and 3,4-dihydroxybenzoate, but this activity was lost upon dialysis. No requirement for a cofactor was found, but rather reduced incubation conditions were necessary to restore enzyme activity. The 4-hydroxylamino-degrading enzyme was purified and the role of this novel type of enzyme in the degradation of nitroaromatic compounds is discussed.

引用

页码：381 / 386

页数：6

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