DIRECT NUCLEOTIDE SEQUENCING USING TOTAL CELLULAR RNA FROM DENGUE-VIRUS-INFECTED MOSQUITO CELLS

被引：2

作者：

KAUTNER, IM

LAM, SK

机构：

[1] Dept. of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur

来源：

RESEARCH IN VIROLOGY | 1992年 / 143卷 / 03期

关键词：

DENGUE; FLAVIVIRIDAE; RNA; ISOLATION; DIRECT NUCLEOTIDE SEQUENCING; DIAGNOSIS; MOSQUITO;

D O I：

10.1016/S0923-2516(06)80103-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In recent years, a large amount of nucleotide sequence data for dengue viruses has been published. Most of it was derived by sequencing cDNA synthesized from highly purified genomic viral RNA. This paper presents a simple and rapid method for the isolation of total RNA from mosquito cells infected with dengue viruses. This RNA can be used for direct nucleotide sequencing with specific primers without the need for further purification.

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页码：193 / 197

页数：5

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