HIGH-SENSITIVE PROTEIN-SEQUENCE ANALYSIS

The primary structure determination of proteins and peptides has made rapid progress in the recent years and became an increasingly important tool of basic and applied research in various areas of biochemistry, molecular biology, medicine and biotechnology. Direct amino acid sequence analysis is frequently applied in combination with mass spectrometry or DNA-sequencing of the protein's gene. Summarized are the essential techniques which facilitate protein purification and sequence analysis in the picomole scale, such as HPLC chromatography, blotting for direct amino acid analysis and N-terminal sequencing. Modern sequencer design is outlined.