HUMAN LIVER L-ALANINE GLYOXYLATE AMINOTRANSFERASE - CHARACTERISTICS AND ACTIVITY IN CONTROLS AND HYPEROXALURIA TYPE-I PATIENTS USING A SIMPLE SPECTROPHOTOMETRIC METHOD

被引：22

作者：

WANDERS, RJA

RUITER, J

VANROERMUND, CWT

SCHUTGENS, RBH

OFMAN, R

JURRIAANS, S

TAGER, JM

机构：

[1] UNIV AMSTERDAM HOSP,DEPT PEDIAT,AMSTERDAM,NETHERLANDS

[2] UNIV AMSTERDAM HOSP,DEPT CLIN CHEM,AMSTERDAM,NETHERLANDS

[3] UNIV AMSTERDAM,BIOCHEM LAB,AMSTERDAM,NETHERLANDS

来源：

CLINICA CHIMICA ACTA | 1990年 / 189卷 / 02期

关键词：

Alanine-glyoxylate aminotransferase; Oxalate; Peroxisomal disorder; Peroxisome; Primary hyperoxaluria type I;

D O I：

10.1016/0009-8981(90)90084-6

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We have studied the characteristics of human liver alanine-glyoxylate aminotransferase, which is deficient in hyperoxaluria type I, an inherited disorder of glyoxylate metabolism. The enzyme was optimally active at pH 8.0 showing apparent Km values for l-alanine and glyoxylate of 8.3 and 1.3 mmol/l, respectively. Activity was found to proceed linearly for up to 4 h. Measurements under these optimal conditions enabled the biochemical diagnosis of hyperoxaluria type I to be made via enzyme activity measurements in percutaneous needle biopsy specimens of liver tissue. © 1990.

引用

页码：139 / 144

页数：6

共 9 条

[1] A NEW MICRO-ASSAY FOR HUMAN-LIVER ALANINE - GLYOXYLATE AMINOTRANSFERASE [J].