SPIN-LATTICE RELAXATION IN THE TRIPLET-STATE OF THE BURIED TRYPTOPHAN RESIDUE OF RIBONUCLEASE-T1

被引：14

作者：

GHOSH, S ^{[1
]}

PETRIN, M ^{[1
]}

MAKI, A ^{[1
]}

机构：

[1] UNIV CALIF DAVIS, DEPT CHEM, DAVIS, CA 95616 USA

来源：

BIOPHYSICAL JOURNAL | 1986年 / 49卷 / 03期

关键词：

D O I：

10.1016/S0006-3495(86)83701-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The individual spin-lattice relaxation (SLR) rate constants (Wij) between the lowest triplet sublevels of the lone tryptophan residue buried in the interior of the globular protein ribonulease T1 have been reported in the temperature range 1.2 to 3.0 K in zero applied magnetic field. The SLR rate constants between spin sublevels exhibit marked anisotropy in their magnitudes and also show appreciable sensitivity to the glycerol content of the aqueous cryogenic matrix. The temperature dependence of SLR suggests that in the temperature range investigated a direct process contributes dominantly to the SLR in this protein.

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页码：753 / 760

页数：8

相关论文

共 26 条

[1] PROTEIN CONFORMATION FROM ELECTRON-SPIN RELAXATION DATA [J].

ALLEN, JP ;

COLVIN, JT ;

STINSON, DG ;

FLYNN, CP ;

STAPLETON, HJ .

BIOPHYSICAL JOURNAL, 1982, 38 (03) :299-310

[2] SPIN-LATTICE RELAXATION IN PHOSPHORESCENT TRIPLET-STATE OF ACRIDINE AND PHENANTHRENE IN ZERO MAGNETIC FIELD [J].

ANTHEUNIS, DA ;

BOTTER, BJ ;

SCHMIDT, J ;

VERBEEK, PJF ;

VANDERWAALS, JH .

CHEMICAL PHYSICS LETTERS, 1975, 36 (02) :225-228

[3] DYNAMICS OF TYROSINE TRIPLET-STATE FROM MAGNETIC RESONANCE-SATURATED PHOSPHORESCENCE DECAY MEASUREMENTS [J].

CO, T ;

HOOVER, RJ ;

MAKI, AH .

CHEMICAL PHYSICS LETTERS, 1974, 27 (01) :5-13

[4] DYNAMICS OF A PROTEIN MATRIX REVEALED BY FLUORESCENCE QUENCHING [J].

EFTINK, MR ;

GHIRON, CA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (09) :3290-3294

[5] FLUORESCENCE QUENCHING AND ISOTOPE EFFECT OF TRYPTOPHAN [J].

EISINGER, J ;

NAVON, G .

JOURNAL OF CHEMICAL PHYSICS, 1969, 50 (05) :2069-&

[6] ANISOTROPIC SATURATION OF ELECTRON SPIN RESONANCE IN PHOTO-EXCITED TRIPLET STATE OF PYRENE-D-10 [J].

FISCHER, PHH ;

DENISON, AB .

MOLECULAR PHYSICS, 1969, 17 (03) :297-&

[7] ANISOTROPIC SPIN-LATTICE RELAXATION IN THE TRIPLET-STATE OF NAPHTHALENE-H8 IN N-PENTANE [J].

GHOSH, S ;

WEERS, JG ;

PETRIN, M ;

MAKI, AH .

CHEMICAL PHYSICS LETTERS, 1984, 108 (01) :87-93

[8] TEMPERATURE-DEPENDENCE OF SPIN-LATTICE RELAXATION RATES IN TRIPLET-STATE OF PYRAZINE AT LOW-TEMPERATURES [J].

HALL, LH ;

ELSAYED, MA .

CHEMICAL PHYSICS, 1975, 8 (03) :272-288

[9]

HERSHBERGER MH, 1984, BIOCHEMISTRY-US, V19, P2204

[10] PHOSPHORESCENCE OF RIBONUCLEASE-T1 IN SOLUTION AT 293K [J].

IMAKUBO, K ;

KAI, Y .

JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN, 1977, 42 (04) :1431-1432