APPLICATION OF A SEMIAUTOMATED SOS CHROMOTEST FOR MEASURING GENOTOXICITIES OF COMPLEX ENVIRONMENTAL MIXTURES CONTAINING POLYCYCLIC AROMATIC-HYDROCARBONS

被引：13

作者：

NYLUND, L ^{[1
]}

HAKALA, E ^{[1
]}

SORSA, M ^{[1
]}

机构：

[1] OULU REG INST OCCUPAT HLTH,OULU,FINLAND

来源：

MUTATION RESEARCH | 1992年 / 276卷 / 1-2期

关键词：

SOS CHROMOTEST; SEMIAUTOMATED; POLYCYCLIC AROMATIC HYDROCARBONS;

D O I：

10.1016/0165-1110(92)90061-D

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Soxhlet-extracted samples of standard reference materials (SRMs) 1649 (PARI: urban dust/organics) and 1650 (PAR2: diesel particulate matter) from the U.S. Institute of Standards and Technology were tested for induction of SOS functions using a semi-automated version of the SOS chromotest with Escherichia coli PQ37. Concentrations of 10 polycyclic aromatic hydrocarbons in the extracts were determined using reversed-phase HPLC. Only the diesel particulate matter (PAR2) extracts expressed SOS induction activity, which decreased when metabolic activation was used. Mutagenic PAH compounds (e.g., chrysene) were found in higher concentrations in the PAR2 extracts than in the PAR1 extracts but this could not explain the genotoxicity while it was mainly exhibited without metabolic activation. The direct genotoxic activity of the diesel particulate matter sample PAR2 is probably caused by nitroaromatic compounds; this was also supported by parallel studies with the Ames/Salmonella assay.

引用

页码：125 / 132

页数：8

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